|Application ||WB, IHC-F, FC, E, IP|
|Calculated MW||50170 Da|
|Other Species||Pig,Channel catfish|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Raw 264 cell line.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor type A, SR-A, CD204, Msr1, Scvr|
|Target/Specificity||Rat anti-Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraseret al.1993). Rat anti-Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daughertyet al.2000).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD204 Antibody, clone 2F8 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.Nature. 364 (6435): 343-6. 2. de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.J Exp Med. 180 (2): 705-9. 3. Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.Eur J Immunol. 25 (2): 466-73. 4. Gordon, S. (1995) The macrophage.Bioessays. 17 (11): 977-86. 5. Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.Immunol Lett. 43 (1-2): 7-14. 6. Rosen, H. and Hughes, D.A. (1995) Assays of Myeloid Cell Function: Migration and adhesion in vivo.Weir Handbook of Experimental Immunology. London, Blackwell Scientific Publications. 5th, ed. In Press. 7. Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.J Lipid Res. 41 (10): 1568-77. 8. Moldenhauer, L.M. et al. (2010) GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice.J Immunol. 185 (11): 7085-96. 9. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.Eur J Cell Biol. 87: 91-9. 10. Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.J Biol Chem. 286: 4854-70. 11. Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.Neurobiol Dis. 42 (3): 221-30. 12. Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.Dis Model Mech. 4: 212-27. 13. Swain, S.D. et al. (2011) Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens.Infect Immun. 79 (5): 1905-14. 14. Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.BMC Immunol. 12: 37. 15. Zaynagetdinov, R et al. (2011) A critical role for macrophages in promotion of urethane-induced lung carcinogenesis.J Immunol. 187 (11): 5703-11. 16. Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.Fish Shellfish Immunol. 15: 169-81. 17. Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.Scand J Immunol. 60: 238-48. 18. Koronyo Y et al. (2015) Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimer's disease.Brain. 138 (Pt 8): 2399-422. 19. Nielsen, B.S. et al. (2008) Matrix metalloproteinase 13 is induced in fibroblasts in polyomavirus middle T antigen-driven mammary carcinoma without influencing tumor progression.PLoS One. 3 (8): e2959. 20. Tao, J. et al. (2015) ClC-3 deficiency prevents atherosclerotic lesion development in ApoE-/-mice.J Mol Cell Cardiol. 87: 237-247. 21. Prins, J.R. et al. (2015) Unstable Foxp3+ Regulatory T Cells and Altered Dendritic Cells Are Associated with Lipopolysaccharide-Induced Fetal Loss in Pregnant IL10-Deficient MiceBiology of Reproduction. Jul 29 [Epub ahead of print]. 22. Almholt, K. et al. (2015) Spontaneous lung and lymph node metastasis in transgenic breast cancer is independent of the urokinase receptor uPAR.Clin Exp Metastasis. 32 (6): 543-54. 23. Verheijden S et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.Glia. 63 (9): 1606-20.
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