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>   home   >   Products   >   Primary Antibodies   >   Anti-Rat CD31 Antibody, clone TLD-3A12 (RPE)   

Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin)

Mouse Anti-Rat Monoclonal Antibody

  • WB - Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) ABD12060
    Published customer image:Mouse anti Rat CD31 antibody, clone TLD-3A12 used for the quantitation of vascular channels in venous thrombi by western blotting.Image caption:Effects of Temporary Dietary Iron Restriction on Leukocyte Recruitment, Neovascularization, and Plasmin System of Stasis-Induced Venous Thrombi in Rats. (A) Representative images of CD68 staining of the venous thrombi. Scale bars: 100 µm. (B) Quantification of CD68 positive cells in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). (C) Representative images of vWF staining of the venous thrombi. Scale bars: 50 µm. Arrows show vascular channels. (D) Quantification of vascular channels in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). The number of CD68 positive cells or vascular channels in 10 randomly selected high power fields (HPF) was counted in a blinded manner. (E) CD31 protein expression (top; representative gel blots depicting expression of CD31 and GAPDH, bottom; relative levels of expression) in the venous thrombi of the DVT and DVT+IR groups (n = 5 in each group). (F) Intrathrombotic gene expression of uPA, tPA, PAI-1, and fibrinogen a chain in the DVT and DVT+IR groups (n = 4 in each group). *p < 0.05 versus the DVT group.From: Oboshi M, Naito Y, Sawada H, Hirotani S, Iwasaku T, Okuhara Y, et al. Temporary Dietary Iron Restriction Affects the Process of Thrombus Resolution in a Rat Model of Deep Vein Thrombosis.PLoS ONE 10: e0126611.
  • IHC-P - Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) ABD12060
    Published customer image:Mouse anti Rat CD31 antibody, clone TLD-3A12 used for detection of ret endothelial cells by immunohistochemistry of paraffin embedded tumor tissue.Image caption:Comparison of tumour microvessel fractal dimension and diameter in control and rhEPO-treated rats. (A, B) Examples of CD31-stained microvessels in the tumour core of control and rhEPO-treated animals. Bar, 25? µm. (C) Microvessel fractal dimension in the tumour core and rim in control and rhEPO-treated animals. Columns, mean; bars, s.e.; *P=0.006. (D) Microvessel diameter in the tumour core and rim in control and rhEPO-treated animals. Columns, mean; bars, s.e.; *P
  • IF - Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) ABD12060
    Published customer image:Mouse anti Rat CD31 antibody, clone TLD-3A12 used for detection of ret endothelial cells by immunofluorescence.Image caption:Evaluation of endothelial cell apoptosis with TUNEL staining in frozen sections. Quantification of TUNEL positive cells showed significantly more TUNEL(+) cells/lesion (A) and TUNEL (+) cells/mm2 (B) with treatment of NP-ATP µ-Raf compared to the control group on day 3 and 5 after laser injury. There was a statistically significant reduction of CNV size noted on day 7(C). Double-immunofluorescent staining of frozen sections obtained at 3, 5 and 7 days after laser photocoagulation for the endothelial cell marker CD31 and TUNEL stain (D). *P
  • IF - Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) ABD12060
    Published customer image:Mouse anti Rat CD31 antibody, clone TLD-3A12 used for the detection of mesenteric vasculature in rats by immunofluorescence.Image caption:(A) Portal-systemic shunting in common bile duct-ligated rats treated with vehicle or brilliant blue G (BBG, CBDL-vehicle: n = 5; CBDL-BBG: n = 7). Compared with vehicle, BBG significantly improved the severity of shunting. (B) Mesenteric vascular density in sham or CBDL rats treated with vehicle or BBG (sham-vehicle: n = 6; sham-BBG: n = 6; CBDL-vehicle: n = 6; CBDL-BBG: n = 9). Vascular area and length per unit of mesenteric window area were significantly reduced in CBDL rats with BBG treatment. ap
  • IHC-P - Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) ABD12060
    Published customer image:Mouse anti RatCD31 antibody, clone TLD-3A12 used for the detection of rat CD31 by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Effects of the PPAR-&delta; agonist GW610742 on angiogenesis.Representative images from the sham-operated , myocardial infarction alone , and MI treated with GW610742 (MI + GW) groups on day 7 (A) and day 14 (B) post-surgery. Images using Masson’s trichrome staining show vessels in the heart post-operation. The immunoreactivity and positive cell numbers for CD31 and Ki-67 were counted in each group post-operation. Values are represented as the mean ± the standard error of the mean . *P &lt; 0.05 and vs. **P &lt; 0.01 the corresponding MI group. Scale bars = 100 &mu;m and 50 &mu;m for MT, CD31, and Ki-67 staining, respectively. Sham (n = 3/each day), MI (n = 7/each day), MI + GW (n = 7/each day).From: Park JR, Ahn JH, Jung MH, Koh J-S, Park Y, Hwang S-J, et al. Effects of Peroxisome Proliferator-Activated Receptor-&delta; Agonist on Cardiac Healing after Myocardial Infarction.PLoS ONE 11(2): e0148510.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q3SWT0
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names TLD-3A12
Calculated MW 76189 Da
Additional Information
Other Species Rh,Pig
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Activated, Lewis rat derived microglial cells.
Shelf Life 18 months from date of despatch.
Gene ID 29583
Other Names Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam
Target/Specificity Mouse anti-Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilsonet al.2007). Mouse anti-Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Macleanet al.2001)
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat.
J. Neurosci. Res. 45(6):747-57. 2. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury.
Am J Pathol. 163: 1587-98. 3. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreaticadult progenitor cell populations.
Stem Cells Dev. 18: 1389-98. 4. Ott, I. et al. (2005) Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction.
FASEB J. 19: 992-4. 5. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction.
J Am Coll Cardiol. 49: 2292-300. 6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation.
J Immunol. 183: 3099-108. 7. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.
J Pharmacol Exp Ther. 286: 447-58. 8. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis.
Arthritis Rheum. 48: 2173-7. 9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development.
Am J Pathol. 157: 647-58. 10. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation.
J Cereb Blood Flow Metab. 30: 1625-36. 11. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model.
Mol Med. 13: 480-7. 12. Nakao, A. et al.(2011)Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts.
Cardiovasc Res. 89: 457-63. 13. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans.
Toxicol Pathol. 35: 904-9. 14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis.
Perit Dial Int. 29: 26-35. 15. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation.
J Pharmacol Exp Ther. 306: 8-12. 16. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues.
J Immunol Methods. 322: 137-42. 17. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation.
J Cereb Blood Flow Metab. 30: 1847-59. 18. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization.
PLoS One. 6: e18864. 19. MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells.
J Neuroimmunol. 118: 223-32. 20. Ceelen, W. et al. (2007) Recombinant human erythropoietin alpha modulates the effects of radiotherapy on colorectal cancer microvessels.
Br J Cancer. 96: 692-700. 21. Tung, H.C. et al. (2015) The Beneficial Effects of P2X7 Antagonism in Rats with Bile Duct Ligation-induced Cirrhosis.
PLoS One. 10 (5): e0124654. 22. Oboshi, M. et al. (2015) Temporary dietary iron restriction affects the process of thrombus resolution in a rat model of deep vein thrombosis.
PLoS One. 10 (5): e0126611. 23. Wu, S.H. et al. (2015) Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury.
Cytotherapy. 17 (8): 1066-75. 24. Ikutomi, M. et al. (2015) Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation.
J Mol Cell Cardiol. 86: 121-35. 25. Ferrantelli Evelina et al. (2016) The dipeptide alanyl-glutamine ameliorates peritoneal fibrosis and attenuates IL-17 dependent pathways during peritoneal dialysis
Kidney International. Jan 8 [Epub ahead of print] 26. Lux, M. et al. (2016) In vitro maturation of large-scale cardiac patches based on a perfusable starter matrix by cyclic mechanical stimulation.
Acta Biomater. 30: 177-87. 27. Liu, T. et al. (2015) A study of the relationship of metabolic MR parameters to estrogen dependence in breast cancer xenografts.
NMR Biomed. 28 (9): 1087-96. 28. Saito, K. et al. (2015) Responses of pulp vasculature after cavity preparation in rat molars
Journal of Oral Biosciences. 57 (3): 157-64. 29. Kakaiy, A. et al. (2015) Comparing protective effect of grape seed extract versus atorvastatin on endometriosis in rat model: Evidence for immunohistochemical and biochemical alterations.
Vet Res Forum. 6 (2): 101-10. 30. Brandl, A. et al. (2014) A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitro.
BMC Cell Biol. 15: 12. 31. Sun, C.K. et al. (2015) Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats.
Am J Transl Res. 7 (2): 209-31. 32. Matsugami, al. (2014) VEGF secretion by adipose tissue-derived regenerative cells is impaired under hyperglycemic conditions via glucose transporter activation and ROS increase
Biomedical Research. 35 (6): 397-405. 33. Park; J.R. et al. (2016) Effects of Peroxisome Proliferator-Activated Receptor-δ Agonist on Cardiac Healing after Myocardial Infarction.
PLoS One. 11 (2): e0148510.

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