|Application ||FC, E|
|Calculated MW||50495 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified human tumour necrosis factor receptor type 1.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor-binding protein 1, TBPI, TNFRSF1A, TNFAR, TNFR1|
|Target/Specificity||Mouse anti-Human CD120a antibody, clone H398 recognizes an extracellular domain of the 55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75 kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes.Mouse anti-Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.Mouse anti-Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thomaet al.1990andDriet al.1999).Mouse anti-Human CD120a antibody, clone H398 is routinely tested in flow cytometry on human peripheral blood monocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human CD120a Antibody, clone H398 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Thoma, B. et al. (1990) Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses.J. Exp. Med. 172: 1019-1023. 2. Menegazzi, R. et al. (1994) Evidence that tumor necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor.Blood 84: 287-293. 3. Dri, P. et al. (1999) Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst.J. Immunol. 162: 460-466. 4. Kohrgruber, N. et al. (1999) Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.J Immunol. 163:3250-3259. 5. Weigart, N. et al. (1996) Gastrin secretion from primary cultures of rabbit antral G cells; stimulation by inflammatory cytokines.Gasteroenterology. 110: 147 - 154. 6. Kennedy, G. et al. (2010) Biochemical and vascular aspects of pediatric chronic fatigue syndrome.Arch Pediatr Adolesc Med. 164: 817-23. 7. Gregory, A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.Nature. 488 (7412): 508-11. 8. Schett, G. et al. (2003) TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.Cell Death Differ. 10: 1126-36. 9. Buckley, C.D. et al. (2005) Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration.J Leukoc Biol.79: 303-11. 10. Thiery, J. et al. (2003) Potentiation of a tumor cell susceptibility to autologous CTL killing by restoration of wild-type p53 function.J Immunol. 170: 5919-26. 11. Lin, K.H. et al. (2009) Mechanisms of resveratrol-induced platelet apoptosis.Cardiovasc Res. 83: 575-85. 12. Baker, P.K. et al. (2012) Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion.Blood. 100: 647-53. 13. Nakamura-Lopez, Y. et al. (2015) RSV P-protein impairs extrinsic apoptosis pathway in a macrophage-like cell line persistently infected with respiratory syncytial virus.Virus Res. 204: 82-7.
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