|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human foreskin fibroblasts.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.In immunohistological studies Mouse anti-Human Fibroblasts/Epithelial Cells antibody.Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al.2001).Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Fearns C & Dowdle EB (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro.Int J Cancer. 50 (4): 621-7. 2. Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.Nephrol Dial Transplant. 15 (11): 1766-72. 3. van Osch, G.J. et al. (2001) Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage.Biochem Biophys Res Commun. 280 (3): 806-12. 4. Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.Biomaterials. 34 (14): 3547-58. 5. Morito, T. et al. (2008) Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.Rheumatology (Oxford). 47 (8): 1137-43. 6. Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.J Cell Mol Med. 15: 525-34. 7. Telfer, J.F. and Brock, J.H. (2002) Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium.Ann Rheum Dis. 61: 741-4. 8. English, A. et al. (2007) A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.Rheumatology (Oxford). 46: 1676-83. 9. Jones, E.A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow.Cytometry B Clin Cytom. 70: 391-9. 10. Nimura, A. et al. (2008) Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum.Arthritis Rheum. 58: 501-10. 11. Miranda-Carús, M.E. et al. (2004) Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active.Arthritis Rheum. 50: 2786-93. 12. Petrow, P.K. et al. (2002) Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis.Ann Rheum Dis. 61: 391-7. 13. Sekiya, I. et al. (2012) Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.J Orthop Res. 30: 943-9. 14. Kanayama, M. et al. (2009) Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis.J Immunol. 182: 8015-25. 15. Pap, T. et al. (2003) Osteoclast-independent bone resorption by fibroblast-like cellsArthritis Res Ther. 5: R163-73. 16. Shi, Y. et al. (2012) Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration.Tissue Eng Part A. 18: 1313-21. 17. Scutt, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.J Orthop Surg Res. 3: 32. 18. Jones. E.A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.Arthritis. Rheum. 46: 3349-60. 19. Schminke, B. et al. (2015) The pathology of bone tissue during peri-implantitis.J Dent Res. 94 (2): 354-61. 20. De Bari, C. et al. (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.Arthritis Rheum. 54 (4): 1209-21. 21. Iyyanki, T. et al. (2015) Harvesting technique affects adipose-derived stem cell yield.Aesthet Surg J. 35 (4): 467-76. 22. Asano, T. et al. (2014) α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis.Rheumatology (Oxford). 53 (3): 415-24. 23. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505. 24. De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.Arthritis Rheum. 58 (1): 240-50. 25. Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129 (3): 163-73. 26. Martinez, C. et al. (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs.Blood. 109 (10): 4245-8. 27. Jayne, D.G. et al. (1999) A three-dimensional in-vitro model for the study of peritoneal tumour metastasis.Clin Exp Metastasis. 17 (6): 515-23. 28. Lu, G. et al. (2013) Transplantation-potential-related biological properties of decidua basalis mesenchymal stem cells from maternal human term placenta.Cell Tissue Res. 352 (2): 301-12. 29. Pandya, J.M. et al. (2016) CD4+ and CD8+ CD28null T cells are Cytotoxic to Autologous Muscle Cells in Polymyositis Patients.Arthritis Rheumatol. Feb 19. [Epub ahead of print] 30. Manoussaka, M.S. et al. (2005) Flow cytometric characterisation of cells of differing densities isolated from human term placentae and enrichment of villous trophoblast cells.Placenta. 26 (4): 308-18. 31. Todisco, E. et al. (2002) CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.Leukemia. 16 (10): 2046-54.
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