|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human foreskin fibroblasts.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.In immunohistological studies Mouse anti-Human Fibroblasts/Epithelial Cells antibody.Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al.2001).Mouse anti-Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fearns C & Dowdle EB (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro.Int J Cancer. 50 (4): 621-7. 2. Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.Nephrol Dial Transplant. 15 (11): 1766-72. 3. van Osch, G.J. et al. (2001) Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage.Biochem Biophys Res Commun. 280 (3): 806-12. 4. Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.Biomaterials. 34 (14): 3547-58. 5. Morito, T. et al. (2008) Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.Rheumatology (Oxford). 47 (8): 1137-43. 6. Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.J Cell Mol Med. 15: 525-34. 7. Telfer, J.F. and Brock, J.H. (2002) Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium.Ann Rheum Dis. 61: 741-4. 8. English, A. et al. (2007) A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.Rheumatology (Oxford). 46: 1676-83. 9. Jones, E.A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow.Cytometry B Clin Cytom. 70: 391-9. 10. Nimura, A. et al. (2008) Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum.Arthritis Rheum. 58: 501-10. 11. Miranda-Carús, M.E. et al. (2004) Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active.Arthritis Rheum. 50: 2786-93. 12. Petrow, P.K. et al. (2002) Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis.Ann Rheum Dis. 61: 391-7. 13. Sekiya, I. et al. (2012) Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.J Orthop Res. 30: 943-9. 14. Kanayama, M. et al. (2009) Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis.J Immunol. 182: 8015-25. 15. Pap, T. et al. (2003) Osteoclast-independent bone resorption by fibroblast-like cellsArthritis Res Ther. 5: R163-73. 16. Shi, Y. et al. (2012) Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration.Tissue Eng Part A. 18: 1313-21. 17. Scutt, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.J Orthop Surg Res. 3: 32. 18. Jones. E.A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.Arthritis. Rheum. 46: 3349-60. 19. Schminke, B. et al. (2015) The pathology of bone tissue during peri-implantitis.J Dent Res. 94 (2): 354-61. 20. De Bari, C. et al. (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.Arthritis Rheum. 54 (4): 1209-21. 21. Iyyanki, T. et al. (2015) Harvesting technique affects adipose-derived stem cell yield.Aesthet Surg J. 35 (4): 467-76. 22. Asano, T. et al. (2014) α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis.Rheumatology (Oxford). 53 (3): 415-24. 23. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505. 24. De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.Arthritis Rheum. 58 (1): 240-50. 25. Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129 (3): 163-73. 26. Martinez, C. et al. (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs.Blood. 109 (10): 4245-8. 27. Jayne, D.G. et al. (1999) A three-dimensional in-vitro model for the study of peritoneal tumour metastasis.Clin Exp Metastasis. 17 (6): 515-23. 28. Lu, G. et al. (2013) Transplantation-potential-related biological properties of decidua basalis mesenchymal stem cells from maternal human term placenta.Cell Tissue Res. 352 (2): 301-12. 29. Pandya, J.M. et al. (2016) CD4+ and CD8+ CD28null T cells are Cytotoxic to Autologous Muscle Cells in Polymyositis Patients.Arthritis Rheumatol. Feb 19. [Epub ahead of print] 30. Manoussaka, M.S. et al. (2005) Flow cytometric characterisation of cells of differing densities isolated from human term placentae and enrichment of villous trophoblast cells.Placenta. 26 (4): 308-18. 31. Todisco, E. et al. (2002) CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.Leukemia. 16 (10): 2046-54.
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