|Calculated MW||70153 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||PHA activated rat lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, CD71, Tfrc, Trfr|
|Target/Specificity||Mouse anti-Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihtonet al.1992).An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).Mouse anti-rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardiet al.2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195kDa under non-reducing conditions reflecting it's homodimeric structure.|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat CD71 Antibody, clone OX-26 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.Immunology 54: 333-341. 2. Yefimova, M.G. et al. (1984) Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons rats. Inv. Opth. & Vis. Sci. 43: 537-545. 3. Jefferies, W.A. et al. (1984) Transferrin receptor on endothelium of brain capillaries.Nature 312: 162-163. 4. Lipardi, C. et al. (2002) Differential recognition of a tyrosine-dependent signal in the basolateral and endocytic pathways of thyroid epithelial cells. Endocrinology. 143: 1291-1301. 5. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreaticadult progenitor cell populations.Stem Cells Dev. 18: 1389-98. 6. Jung, S.H. et al. (2008) Plantaris muscle of aged rats demonstrates iron accumulation and altered expression of iron regulation proteins.Exp Physiol. 93: 407-14. 7. Chen, X. et al. (2000) Oxidative damage in an esophageal adenocarcinoma model with rats.Carcinogenesis. 21: 257-63. 8. Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.Acta Histochem Cytochem. 42: 129-36. 9. Petrusca, D.N. et al. (2010) Sphingolipid-mediated inhibition of apoptotic cell clearance by alveolar macrophages.J Biol Chem. 285: 40322-32. 10. Wu, Y.J. et al. (2007) In vivo leukocyte labeling with intravenous ferumoxides/protamine sulfate complex and in vitro characterization for cellular magnetic resonance imaging.Am J Physiol Cell Physiol. 293: C1698-708. 11. Yefimova M.G. et al. (2002) Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons rats.Invest Ophthalmol Vis Sci. 43: 537-45. 12. Gosk, S. et al. (2004) Targeting anti-transferrin receptor antibody (OX26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion.J Cereb Blood Flow Metab. 24: 1193-204. 13. Pang, Z. (2008) Preparation and brain delivery property of biodegradable polymersomes conjugated with OX26.J Control Release. 128: 120-7. 14. Moos, T, Morgan, E.H. (2001) Restricted transport of anti-transferrin receptor antibody (OX26) through the blood-brain barrier in the rat.J Neurochem. 79: 119-29. 15. Moos, T. et al. (2003) Delivery of transferrin and immunoglobulins to the ventricular system of the rat.Front Biosci. 8: a102-9. 16. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.Blood. 109: 5223-9. 17. Rathnasamy, G. et al.et al. (2011) Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen/Nitrogen Species J. Neurosci 31: 17982-95. 18. De Luca, M.A. et al. (2015) Lactoferrin- and antitransferrin-modified liposomes for brain targeting of the NK3 receptor agonist senktide: Preparation and in vivo evaluation.Int J Pharm. 479: 129-137.
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