|Application ||FC, IP|
|Calculated MW||82522 Da|
|Other Species||Cynomolgus Pr,Rh|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Other Names||Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1|
|Target/Specificity||Mouse anti-Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Mulleret al.1989).CD31 is also known as PECAM-1.The binding epitope for mouse anti-human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcettet al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD31 Antibody, clone WM59 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Carbanas. C., Sanchez-Madrid, F., et al. (1989) Characterisation of a novel myeloid antigen regulated during differentiation of monocytic cells.Eur J. Immunol. 19: 1373-1378. 2. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. 3. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.J. Immunol. 145: 3889-3897. 4. DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.Immunol. Today 15: 490-495. 5. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.J Pharmacol Exp Ther 321: 656-662. 6. Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31adhesion in the formation of interendothelial cell contacts.J Cell Biol. 128:1229-41. 7. Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.J Leukoc Biol. 82: 1278-88. 8. Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.Clin Immunol. 114: 154-63. 9. Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.J Cell Biol. 148: 1151-8. 10. Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.BMC Cardiovasc Disord. Sep; 10:43. 11. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.Clin Exp Immunol. 130: 256-62. 12. Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.Anal Chem. 87 (6): 3239-46. 13. Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.J Clin Pathol. 57 (9): 965-9. 14. Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.J Clin Pathol. 56 (10): 736-41. 15. Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.Biomed Rep. 3 (5): 626-636. 16. Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.Stem Cells Dev. 23 (22): 2730-43. 17. Fabre-Mersseman V et al. (2011) CD4? recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.AIDS. 25 (9): 1153-62. 18. Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.Blood. 111 (10): 5173-81. 19. Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.J Immunol. 173 (1): 230-5. 20. Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.J Invest Dermatol. 135 (1): 130-41. 21. Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.Br J Haematol. 169 (4): 552-64. 22. Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.Nat Commun. 6: 8009. 23. Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.Am J Pathol. 185 (9): 2563-74. 24. Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.Ann Neurol. 79 (2): 217-30.
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