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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Dog CD3 Antibody, clone CA17.2A12    

Anti-Dog CD3 Antibody, clone CA17.2A12 (FITC)

Mouse Anti-Dog Monoclonal Antibody

  • FC - Anti-Dog CD3 Antibody, clone CA17.2A12 (FITC) ABD12115
    Published customer image:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7: e33274.
  • FC - Anti-Dog CD3 Antibody, clone CA17.2A12 (FITC) ABD12115
    Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD3
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27597
Reactivity Dog
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CA17.2A12
Calculated MW 22642 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
Shelf Life 18 months from date of despatch.
Gene ID 442981
Other Names T-cell surface glycoprotein CD3 epsilon chain, CD3e, CD3E
Target/Specificity Mouse anti-Dog CD3 antibody, clone CA17.2A12 recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti-Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalcoet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Dog CD3 Antibody, clone CA17.2A12 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 2000 Nov;37:637-46. 2. Moore, P. F., Affolter, V. K. and Vernau, W. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
Vet Pathol. 2006 Sep;43:632-45. 3. Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
Vet Immunol Immunopathol. 69:145-64. 4. Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
Vet Immunol Immunopathol. 71:181-95. 5. Byrne, K. et al (2000) A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes.
Vet Immunol Immunopathol. 73:167-82. 6. Machado, G.F. et al. (2011) Intravascular Lymphomatosis in the Central Nervous System of Dogs: Immunohistochemical Investigation in Two Cases
Braz J Vet Pathol 4: 47-51 7. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-relatedmolecules and cytokines in dogs.
Vet Immunol Immunopathol. 140: 175-80. 8. Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.
Vet Immunol Immunopathol. 142: 189-200. 9. Hsiao, Y.W. et al (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
J Immunol. 172: 1508-14. 10. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
J Leukoc Biol. 84: 1501-10. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105: 499-508. 12. Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
J Nutr. 132: 1652S-4S. 13. Hai, M. et al. (2008) Potential genotoxicity from integration sites in CLAD dogs treated successfully with gammaretroviral vector-mediated gene therapy.
Gene Ther. 15: 1067-71. 14. Altmann, S. et al. (2008) High Mobility Group Box 1-Protein expression in canine haematopoietic cells and influence on canine peripheral blood mononuclear cell proliferative activity
Vet Immunol Immunopathol. 126: 367-72. 15. Ting-De Ravin, S.S. et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.
Blood. 107: 3091-7. 16. Miranda, S. et al. (2007) Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol.
Vet Parasitol. 144: 251-60. 17. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. 18. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
Vet Comp Oncol. 12: 29-36.

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Cat# ABD12115
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