|Calculated MW||22642 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, CD3e, CD3E|
|Target/Specificity||Mouse anti-Dog CD3 antibody, clone CA17.2A12 recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti-Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalcoet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Dog CD3 Antibody, clone CA17.2A12 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.Vet Pathol. 2000 Nov;37:637-46. 2. Moore, P. F., Affolter, V. K. and Vernau, W. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.Vet Pathol. 2006 Sep;43:632-45. 3. Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.Vet Immunol Immunopathol. 69:145-64. 4. Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.Vet Immunol Immunopathol. 71:181-95. 5. Byrne, K. et al (2000) A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes.Vet Immunol Immunopathol. 73:167-82. 6. Machado, G.F. et al. (2011) Intravascular Lymphomatosis in the Central Nervous System of Dogs: Immunohistochemical Investigation in Two CasesBraz J Vet Pathol 4: 47-51 7. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-relatedmolecules and cytokines in dogs.Vet Immunol Immunopathol. 140: 175-80. 8. Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.Vet Immunol Immunopathol. 142: 189-200. 9. Hsiao, Y.W. et al (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.J Immunol. 172: 1508-14. 10. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.Immunology. 105: 499-508. 12. Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.J Nutr. 132: 1652S-4S. 13. Hai, M. et al. (2008) Potential genotoxicity from integration sites in CLAD dogs treated successfully with gammaretroviral vector-mediated gene therapy.Gene Ther. 15: 1067-71. 14. Altmann, S. et al. (2008) High Mobility Group Box 1-Protein expression in canine haematopoietic cells and influence on canine peripheral blood mononuclear cell proliferative activityVet Immunol Immunopathol. 126: 367-72. 15. Ting-De Ravin, S.S. et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.Blood. 107: 3091-7. 16. Miranda, S. et al. (2007) Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol.Vet Parasitol. 144: 251-60. 17. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.J Neuroinflammation. 9: 226. 18. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.Vet Comp Oncol. 12: 29-36.
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