|Application ||WB, IHC-P, IHC-F, FC|
|Calculated MW||39336 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Immunogen||C5aR - peptide: Met1 - Asn31|
|Shelf Life||18 months from date of despatch.|
|Other Names||C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88, C5AR1, C5AR, C5R1|
|Target/Specificity||Mouse anti-Human CD88 antibody, clone P12/1 recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31).Mouse anti-Human CD88 antibody, clone P12/1 does not inhibit the binding of C5a to its receptor.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD88 Antibody, clone P12/1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Opperman, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domainJ. Immunol. 151: 3785 - 3794. 2. Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity.In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P: 955-956. 3. Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin. In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P.: 957-959. 4. Oppermann, M. (1995) Cluster report: (C5a receptor). In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. 953-954. 5. Oppermann, M. and Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptorsImmunology. 82: 516-521. 6. Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.J Immunol. 157: 1729-35. 7. Morris, A.C. et al. (2011) C5a-mediated neutrophil dysfunction is RhoA-dependent and predicts infection in critically ill patients.Blood. 117: 5178-88. 8. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.J Biol Chem. 280: 37503-15. 9. Nitta, H. et al. (2013) Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a receptor (CD88).Clin Cancer Res. 19 (8): 2004-13. 10. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.Atherosclerosis. 238 (2): 289-95. 11. Huber-Lang M (2005) Changes in the Novel Orphan, C5a Receptor (C5L2), during Experimental Sepsis and Sepsis in HumansJ Immunol. 174:1104-1110 12. Unnewehr H et al. (2013) Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.J Immunol. 190 (8): 4215-25. 13. Visser T et al. (2011) Isolated blunt chest injury leads to transient activation of circulating neutrophils.Eur J Trauma Emerg Surg. 37 (2): 177-184. 14. Visser T et al. (2012) Homology in systemic neutrophil response induced by human experimental endotoxemia and by trauma.Shock. 37 (2): 145-51. 15. Pollok-Kopp B et al. (2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334.J Biol Chem. 282 (7): 4345-53.
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