|Application ||WB, FC, IP|
|Calculated MW||84782 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Peripheral blood mononuclear cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7|
|Target/Specificity||Mouse anti-Human CD18 (Activation Epitope) antibody, clone MEM-148 recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti-Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD18 (Activation Epitope) Antibody, clone MEM-148 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Drbal, K. et al. (2001) A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 98: 1561-1566. 2. Luissint, A-C.et al. (2008) JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.J. Cell. Biol. 183: 1159-1173. 3. Feng, C. et al. (2011) Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.J Leukoc Biol. 90: 313-21. 4. Anogianaki, A. et al. (2007) Capsaicin an irritant anti-inflammatory compound.J Biol Regul Homeost Agents. 21: 1-4. 5. Arthos, J. et al. (2008) HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.Nat Immunol. 9: 301-9. 6. Cairo, C.W. et al. (2006) Cytoskeletal regulation couples LFA-1 conformational changes to receptor lateral mobility and clustering.Immunity. 25: 297-308. 7. Tang, X.Y. et al. (2008) Intercellular adhesion molecule-3 binding of integrin alphaL beta2 requires both extension and opening of the integrin headpiece.J Immunol. 180: 4793-804. 8. Kudlová M. et al. (2007) Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.Acta Medica (Hradec Kralove). 50: 187-93. 9. Solovjov, D.A. et al. (2005) Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2.J Biol Chem. 280: 1336-45. 10. Ehirchiou, D. et al. (2005) Dual function for a unique site within the beta2I domain of integrin alphaMbeta2.J Biol Chem. 280: 8324-31. 11. Cheng, M. et al. (2007) Mutation of a conserved asparagine in the I-like domain promotes constitutively active integrins alphaLbeta2 and alphaIIbbeta3.J Biol Chem. 282: 18225-32. 12. Shi, M. et al. (2007) A structural hypothesis for the transition between bent and extended conformations of the leukocyte beta2 integrins.J Biol Chem. 282: 30198-206.
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