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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD25 Antibody, clone MEM-181 (RPE)   

Anti-Human CD25 Antibody, clone MEM-181

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD25 Antibody, clone MEM-181  ABD12172
    Published customer image:Potency of Treg-suppression is dependent on strength of stimulus and on Effector:Treg cell (E:T) ratio. (A) Typical FACS plot displaying purity of effector (CD4+CD25-) and Treg (CD4+CD25+) populations in healthy control subjects. (B) Mean counts per minute (CPM) (+SEM) uptake of tritiated thymidine in CD25- effector T cells from healthy controls (N = 27) stimulated with mitogenic CD3/28 beads at 2:1 and 0.2:1 bead: cell ratios. (C) Mean CPM (+SEM) in CD25- effectors v CD25+ Treg cells stimulated with mitogenic CD3/28 beads at 2:1 bead: cell ratio. (D) Mean (+SEM) percentage suppression of effector cell proliferation following activation with CD3/28 beads at 2:1 (closed boxes) and 0.2:1 (open boxes) bead: cell ratios.From: Thorborn G, Pomeroy L, Isohanni H, Perry M, Peters B, et al. Increased Sensitivity of CD4+ T-Effector Cells to CD4+CD25+ Treg Suppression Compensates for Reduced Treg Number in Asymptomatic HIV-1 Infection. PLoS ONE 5: e9254.
  • FC - Anti-Human CD25 Antibody, clone MEM-181  ABD12172
    Staining of PHA stimulated human lymphocyte cells with Mouse anti Human CD25
  • FC - Anti-Human CD25 Antibody, clone MEM-181  ABD12172
    Staining of PHA stimulated human peripheral blood lymphocytes with Mouse anti Human CD25:FITC
  • FC - Anti-Human CD25 Antibody, clone MEM-181  ABD12172
    Staining of PHA stimulated human peripheral blood lymphocytes with Mouse anti Human CD25: RPE- Alexa Fluor® 488
  • FC - Anti-Human CD25 Antibody, clone MEM-181  ABD12172
    Published customer image:Detection of circulating CD4+CD25low/-GITR+cells in SLE patients.(A) Flow-cytometry analysis of anti-GITR and anti-CD25-stained CD4+ T lymphocytes, freshly isolated from blood of a representative SLE patient, reveals the presence of a CD25-GITR+ cell subpopulation. Left panel: gating strategy. Gated CD4+ T lymphocytes were stained with isotype control Abs (middle panel) or anti-GITR and anti-CD25 Abs (right panel). (B) the levels of CD25 expression were evaluated in the upper left and lower left quadrants and MFI is reported. (C) mRNA expression of CD25 (FAM6 fluorochrome) in the indicated subpopulations was evaluated in quadruplicate with real-time PCR. In the same tube, expression of the housekeeping gene HPRT1 (VIC fluorochrome) was evaluated for normalization. Values of CD25 expression (striped column, CD25highGITR-; black column, CD25low/-GITR+) is shown as fold increase of mRNA levels in the positively sorted subpopulations over mRNA levels in effector (CD4+CD25-GITR-) T cells, arbitrarily set equal to 1. Data shown are mean?±?SD of four SLE patients. ***P
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01589
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-181
Calculated MW 30819 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Human PHA blasts; day 3 of culture.
Shelf Life 18 months from date of despatch.
Gene ID 3559
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
Target/Specificity Mouse anti-Human CD25, clone MEM-181 recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Normanet al. 1991) located within its extracellular domain.The IL-2 receptor exists in three forms. A high affinity form consiting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attiaet al.2010). Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwaraet al.2013).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD25 Antibody, clone MEM-181 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Prager, E. et al. (2001) Induction of Hyporesponsiveness and Impaired T lymphocyte activation by the CD31 receptor: ligand pathway in T cells.
J. Immunol. 166: 2364-2371. 2. Thorborn, G. et al. (2010) Increased sensitivity of CD4+ T-effector cells to CD4+CD25+ Treg suppression compensates for reduced Treg number in asymptomatic HIV-1 infection.
PLoS One. 5: e9254. 3. Cutler, A.J. et al. (2010) Umbilical cord-derived mesenchymal stromal cells modulate monocyte function to suppress T cell proliferation.
J Immunol. 185: 6617-23. 4. Lawson, J.M. et al. (2008) Increased resistance to CD4+CD25hi regulatory T cell-mediated suppression in patients with type 1 diabetes.
Clin Exp Immunol. 154: 353-9. 5. Holderness, J. et al. (2007) Select plant tannins induce IL-2Ralpha up-regulation and augment cell division in gammadelta T cells.
J Immunol. 179: 6468-78. 6. Zhang, Y. et al. (2013) Accelerated In Vivo Proliferation of Memory Phenotype CD4+ T-cells in Human HIV-1 Infection Irrespective of Viral Chemokine Co-receptor
PLoS Pathog 9(4): e1003310. 7. Nocentini, G. et al. (2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.
Arthritis Res Ther. 16: 444.

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Cat# ABD12172
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