|Calculated MW||30819 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Human PHA blasts; day 3 of culture.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA|
|Target/Specificity||Mouse anti-Human CD25, clone MEM-181 recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Normanet al. 1991) located within its extracellular domain.The IL-2 receptor exists in three forms. A high affinity form consiting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attiaet al.2010). Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwaraet al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD25 Antibody, clone MEM-181 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Prager, E. et al. (2001) Induction of Hyporesponsiveness and Impaired T lymphocyte activation by the CD31 receptor: ligand pathway in T cells. J. Immunol. 166: 2364-2371. 2. Thorborn, G. et al. (2010) Increased sensitivity of CD4+ T-effector cells to CD4+CD25+ Treg suppression compensates for reduced Treg number in asymptomatic HIV-1 infection.PLoS One. 5: e9254. 3. Cutler, A.J. et al. (2010) Umbilical cord-derived mesenchymal stromal cells modulate monocyte function to suppress T cell proliferation.J Immunol. 185: 6617-23. 4. Lawson, J.M. et al. (2008) Increased resistance to CD4+CD25hi regulatory T cell-mediated suppression in patients with type 1 diabetes.Clin Exp Immunol. 154: 353-9. 5. Holderness, J. et al. (2007) Select plant tannins induce IL-2Ralpha up-regulation and augment cell division in gammadelta T cells.J Immunol. 179: 6468-78. 6. Zhang, Y. et al. (2013) Accelerated In Vivo Proliferation of Memory Phenotype CD4+ T-cells in Human HIV-1 Infection Irrespective of Viral Chemokine Co-receptor PLoS Pathog 9(4): e1003310. 7. Nocentini, G. et al. (2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.Arthritis Res Ther. 16: 444.
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