|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Sheep CD45R antibody, clone 20.96 recognises the ovine CD45R cell surface antigen, a ~220 kDa glycoprotein, expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Sheep CD45R Antibody, clone 20.96 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55. 2. Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.J Virol. 86 (4): 2056-66. 3. Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6.Vet Immunol Immunopathol. 39: 193-9. 4. Lee, C.S. et al. (1988) Identification of a unique lymphocyte subpopulation in the sheep uterus.Immunology. 63: 157-64. 5. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.Nat Immunol. 6: 889-94. 6. Sedgmen, B.J. et al. (2002) Cellular and molecular characterisation of the ovine rectal mucosal environment.Vet Immunol Immunopathol. 86: 215-20. 7. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.PLoS One. 7: e30430. 8. Boppana, D.K. et al. (2005) Cellular infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicumticks.Parasitology. 131: 657-67. 9. Leemans, I. et al. (2001) Comparative studies on surface phenotypes of Theileria lestoquardi and T. annulata schizont-infected cells.Parasitol Res. 87: 768-77. 10. Tanaka, Y. et al. (2008) Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation.Stem Cells Dev. 17: 367-81.
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