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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse CD206 Antibody, clone MR5D3 (RPE)   

Anti-Mouse CD206 Antibody, clone MR5D3

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD206 Antibody, clone MR5D3  ABD12204
    Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor® 647
  • IHC - Anti-Mouse CD206 Antibody, clone MR5D3  ABD12204
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power
  • WB - Anti-Mouse CD206 Antibody, clone MR5D3  ABD12204
    Published customer image:Rat anti Mouse CD206 antibody, clone MR5D3 used for the evaluation of mannose expression by primary microglia from wild type and IL-1 knockout mice by western blotting of isolated microglial lysates.Image caption:Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1&beta;. (A) Representative western blotting data of primary microglial cells produced from wild-type and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1&beta;. Each lane expected to CD206 blotting were applied 7 µg of reduced samples. Non-reduced samples (5 &mu;g) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1&beta; and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1&beta; and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1&beta;. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1&beta;. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1&beta;; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean ± SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1&beta; for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury.J Neuroinflammation. 2012 Apr 7;9:65.
  • IF - Anti-Mouse CD206 Antibody, clone MR5D3  ABD12204
    Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power
  • FC - Anti-Mouse CD206 Antibody, clone MR5D3  ABD12204
    Published customer image:Rat anti Mouse CD206 antibody, clone MR5D3 used for the evaluation of alternative macrophage activation in the lungs of fibrotic IFN&gamma; knockout and wild type mice by flow cytometry on isolated cells.Image caption:Elevated levels of alternative macrophage activation are observed in lung of fibrotic IFN&gamma;R-/- C57Bl/6 mice. 8–12 week old C57Bl/6 IFN&gamma;R-/- mice were intranasally infected with 1x105 pfu MHV68 (WT or M1st) and sacrificed at indicated times post infection (n = 3–4 mice/group at each timepoint). Whole lungs were harvested and assessed for macrophage population and phenotype. (A-B) Absolute number of alveolar and interstitial macrophages were quantified and assessed for alternative activation using RELM&alpha; and CD206 expression.From: O&#39;Flaherty BM, Matar CG, Wakeman BS, Garcia A, Wilke CA, Courtney CL, et al. CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice.PLoS ONE 10(8): e0135719.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61830
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names MR5D3
Calculated MW 164981 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Chimaeric CRD4-7-Fc protein.
Shelf Life 18 months from date of despatch.
Gene ID 17533
Other Names Macrophage mannose receptor 1, MMR, CD206, Mrc1
Target/Specificity Rat anti-mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells (Zamzeet al.2002).The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Suet al.2005).Rat anti-mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamzeet al.2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomareset al.2003andSuet al.2005).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD206 Antibody, clone MR5D3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Martinez-Pomares, L. et al. (2003) Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies.
J Leukoc Biol. 73 (5): 604-13. 2. Zamze, S. et al. (2002) Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor.
J Biol Chem. 277 (44): 41613-23. 3. Hassan, M.F. et al. (2006) The Schistosoma mansoni hepatic egg granuloma provides a favorable microenvironment for sustained growth of Leishmania donovani.
Am J Pathol. 169: 943-53. 4. Hardison, S.E. et al. (2010) Interleukin-17 Is Not Required for Classical Macrophage Activation in a Pulmonary Mouse Model of Cryptococcus neoformansInfection.
Infect Immun. 78: 5341-51. 5. Geier, H. & Celli, J. (2011) Phagocytic receptors dictate phagosomal escape and intracellular proliferation of Francisella tularensis.
Infect Immun. 79 (6): 2204-14. 6. Bacci, M. et al. (2009) Macrophages are alternatively activated in patients with endometriosis and required for growth and vascularization of lesions in a mouse model of disease.
Am J Pathol. 175: 547-56. 7. Chavele, K.M. et al. (2010) Mannose receptor interacts with Fc receptors and is critical for the development of crescentic glomerulonephritis in mice.
J Clin Invest. 120: 1469-78. 8. deSchoolmeester, M.L. et al. (2009) The mannose receptor binds Trichuris muris excretory/secretory proteins but is not essential for protective immunity.
Immunology. 126: 246-55. 9. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. 10. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4(5):e689. 11. Hardison, S.E. et al. (2010) Pulmonary infection with an interferon-gamma-producing Cryptococcus neoformans strain results in classical macrophage activation and protection.
Am J Pathol. 176: 774-85. 12. Hawkes, C.A. et al. (2009) Selective targeting of perivascular macrophages for clearance of beta-amyloid in cerebral amyloid angiopathy.
Proc Natl Acad Sci U S A.106: 1261-6. 13. Zehner, M. et al. (2011) Mannose receptor polyubiquitination regulates endosomal recruitment of p97 and cytosolic antigen translocation for cross-presentation.
Proc Natl Acad Sci U S A. 108: 9933-8. 14. Famulski, K.S. et al. (2010) Alternative macrophage activation-associated transcripts in T-cell-mediated rejection of mouse kidney allografts.
Am J Transplant. 2010 Mar;10(3): 490-7. 15. Takagi, H. et al. (2009) Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages.
Glycobiology. 19: 258-66. 16. Deepe, G.S. Jr. and Buesing, W.R. (2011) Deciphering the Pathways of Death of Histoplasma capsulatum-Infected Macrophages: Implications for the Immunopathogenesis of Early Infection.
J Immunol. 188: 334-44. 17. Schneider, D. et al. (2012) Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness.
J Immunol. 188 (6): 2894-904. 18. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.
J Neurosci. 31: 3610-24. 19. Joyce, K.L. et al. (2012) Using eggs from Schistosoma mansoni as an in vivo model of helminth-induced lung inflammation.
J Vis Exp. 2012: e3905. 20. Su, Y. et al. (2005) Glycosylation influences the lectin activities of the macrophage mannose receptor.
J Biol Chem. 280:32811-20. 21. Verheijden, S. et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.
Glia. 63 (9): 1606-20. 22. O'Flaherty, B.M. et al. (2015) CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice.
PLoS One. 10 (8): e0135719.

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