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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD226 Antibody, clone DX11    

Anti-Human CD226 Antibody, clone DX11

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Published customer image:Mouse anti Human CD226 antibody, clone DX11 used for blocking DNAM-1 on human NK cells to evaluate the role of DNAM-1 in NK cell mediated NK cytotoxicity. Image caption:NO exposed SKO-007 cells enhances NK cell-mediated cytotoxicity. A) NK cells prepared from PBMCs of healthy donors, were incubated with SKO-007 cells, untreated or treated with DETA-NO for 48 h, and used as target cells in a degranulation assay. The assay was performed at the effector:target (E:T) ratio of 2.5:1. After 2 hours at 37°C, cells were stained with anti-CD56, anti-CD3 and anti-CD107a mAbs. Cell surface expression of CD107a was analyzed on CD56+CD3- cells. In order to evaluate the role of DNAM-1, the assay was performed in parallel treating NK cells with blocking anti-DNAM-1 antibody. Results are representative of one out of three independent experiments. B) The MFI of CD107 were calculated based on at least three independent experiments and evaluated by paired Student t test (*P?
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226 : Low Endotoxin
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:RPE
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:FITC
  • FC - Anti-Human CD226 Antibody, clone DX11  ABD12214
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:Azide Free
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15762
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DX11
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Human cytotoxic T lymphocyte clone.
Shelf Life 18 months from date of despatch.
Other Names CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1
Target/Specificity Mouse anti-Human CD226 antibody, clone DX11 recognizes human CD226, a ~65 kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes.Mouse anti-Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojimaet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD226 Antibody, clone DX11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes.
Immunity. 4 (6): 573-81. 2. Shibuya, A. et al. (1998) Protein kinase C is involved in the regulation of both signaling and adhesion mediated by DNAX accessory molecule-1 receptor.
J Immunol. 161 (4): 1671-6. 3. Kojima, H. et al. (2003) CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells.
J Biol Chem. 278 (38): 36748-53. 4. Manes, T.D. and Pober, J.S. (2011) Identification of Endothelial Cell Junctional Proteins and Lymphocyte Receptors Involved in Transendothelial Migration of Human Effector Memory CD4+ T Cells.
J Immunol. 186: 1763-8. 5. Ardolino, M. et al. (2011) DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Blood. 117: 4778-86. 6. Soriani, A. et al. (2009) ATM-ATR-dependent up-regulation of DNAM-1 and NKG2D ligands on multiple myeloma cells by therapeutic agents results in enhanced NK-cell susceptibility and is associated with a senescent phenotype.
Blood. 113: 3503-11. 7. Matusali, G. et al. (2012) The Human Immunodeficiency Virus Type 1 Nef and Vpu Proteins Downregulate the Natural Killer Cell-Activating Ligand PVR.
J Virol. 86: 4496-504. 8. Fionda, C. et al. (2015) Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.
BMC Cancer. 15 (1): 17.

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Cat# ABD12214
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