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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig SLA Class I Antibody, clone JM1E3    

Anti-Pig SLA Class I Antibody, clone JM1E3 (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig SLA Class I Antibody, clone JM1E3 (FITC) ABD12217
    Published customer image:Mouse anti Pig SLA Class I antibody, clone JM1E3 used for the evaluation of SLA Class I expression on bone marrow derived mesenchymal stem cells by flow cytometry.Image caption:Effect of pericardial fluid on pBM-MSCs phenotype. The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.From: Citation: Blázquez R, Sánchez-Margallo FM, Crisístomo V, Báez C, Maestre J, García-Lindo M, et al. Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis. PLoS ONE 10: e0122377.
  • FC - Anti-Pig SLA Class I Antibody, clone JM1E3 (FITC) ABD12217
    Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine SLA 1:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names JM1E3
Additional Information
Other Species H
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Porcine peripheral blood mononuclear cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galianiet al.2002)The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. Mouse anti-pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galianiet al.2002).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig SLA Class I Antibody, clone JM1E3 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Galiani, D. et al.. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors.
In Leucocyte Typing VII. Edited by Mason. D. et al.. Oxford University Press pp 437-39. 2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the Mand N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. 3. Hurtado, C. et al. (2011) The African swine fever virus lectin EP153R modulates the surface membrane expression of MHC class I antigens.
Arch Virol. 156: 219-34. 4. Ding, G. et al. (2010) Suppression of T cell proliferation by root apical papilla stem cells in vitro.
Cells Tissues Organs. 191: 357-64. 5. Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
J Microbiol Biotechnol. 18: 1709-16. 6. Van Parys, A. et al. (2012) Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages.
Vet Res. 43: 52. 7. Löndt, B.Z. et al. (2013) Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organ cultures following adaptation by in vitropassage.
Virus Res. pii: S0168-1702(13)00307-9. 8. Blázquez R et al. (2015) Intrapericardial administration of mesenchymal stem cells in a large animal model: a bio-distribution analysis.
PLoS One. 10 (3): e0122377. 9. Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. : .1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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