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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse Dectin-1 Antibody, clone 2A11 (RPE)   

Anti-Mouse Dectin-1 Antibody, clone 2A11

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse Dectin-1 Antibody, clone 2A11  ABD12226
    Published customer image:Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/MØ. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean±95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (‘C’) and the use of different fungal particle used (‘F’) were assessed by Two-way ANOVA (‘I’ = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7: e45781.
  • FC - Anti-Mouse Dectin-1 Antibody, clone 2A11  ABD12226
    Staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor: RPE
  • FC - Anti-Mouse Dectin-1 Antibody, clone 2A11  ABD12226
    Staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor:FITC
  • IHC-P - Anti-Mouse Dectin-1 Antibody, clone 2A11  ABD12226
    Immunoperoxidase staining of a mouse skin cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power
  • IHC - Anti-Mouse Dectin-1 Antibody, clone 2A11  ABD12226
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6QLQ4
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names 2A11
Calculated MW 27420 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
Shelf Life 18 months from date of despatch.
Other Names C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1, Clec7a, Bgr, Clecsf12, Dectin1
Target/Specificity Rat anti-Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Rat anti-Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse Dectin-1 Antibody, clone 2A11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Brown, G. D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
J. Exp. Med. 196:407-412. 2. Taylor, P. R. et al. (2002) The beta-glucan receptor, Dectin-1 is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.
J. Immunol. 169:3876-3882. 3. Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
J. Leuk. Biol. 76:86-94. 4. Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
Blood 106:2543-2550. 5. Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida Elimination
PLoS One. 5(9):e12828. 6. Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.
Proc Natl Acad Sci U S A. 108 (13): 5360-5. 7. Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.
J Biol Chem. 286: 7822-9. 8. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
FASEB J. 25 (6): 1972-82. 9. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4: e689. 10. Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
PLoS Pathog. 6: e1000714. 11. Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.
Cancer Res. 68: 450-6. 12. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24. 13. Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.
J Infect Dis. 198: 176-85. 14. Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using"designer"microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.
J Biol Chem. 281: 5771-9. 15. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97. 16. Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.
J Exp Med. 208 (8): 1695-705. 17. Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-a production.
Int Immunol. 24 (2): 89-96. 18. Fransen, F. et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
Immunity. pii: S1074-7613(15)00343-X.

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