|Application ||WB, IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Normal murine bone marrow cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g|
|Target/Specificity||Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a 21–25kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Leeet al.2013). Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleminget al.1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendraet al.2007)The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo(Czuprynski, C.J. et al1994,Daleyet al.2008).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Gr-1 Antibody, clone RB6-8C5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.J Immunol. 151 (5): 2399-408. 2. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.J Immunol. 147 (1): 22-8. 3. Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenesinfection.J Immunol. 152 (4): 1836-46. 4. Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.J Immunol. 185 (11): 7057-66. 5. Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.J Pharmacol Exp Ther. 336: 381-90. 6. Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.Blood.117: 1734-44. 7. Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Gué>rin immunotherapy of bladder cancer and orchestrate local immune responses.Cancer Res. 66: 8250-7. 8. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.PLoS Pathog. 3: e193. 9. Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.Endocrinology. 152: 3155-64. 10. Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.J Exp Med. 179:259-68. 11. Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.J Surg Res. 187 (2): 559-70. 12. Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.J Histochem Cytochem. 59: 813-25. 13. Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.J Immunol.190: 340-8. 14. Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.Infect Immun. 82 (1): 253-64. 15. Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.Am J Pathol. 185 (6): 1686-98. 16. Lee, Y.S. et al. (2015) Interleukin-1 signaling in intestinal stromal cells controls KC/CXCR1 secretion, which correlates with the recruitment of IL-22-secreting neutrophils at early stages of Citrobacter rodentiuminfection.Infect Immun. pii: IAI.00670-15. 17. Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.Eur J Immunol. 32 (11): 3235-45.
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