|Application ||IHC-F, FC, IP|
|Calculated MW||27820 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||B7-DC transfected RAW264.7 cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC, CD273, Pdcd1lg2, B7dc, Btdc, Cd273, Pdl2|
|Target/Specificity||Rat anti-Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.Rat anti-Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumotoet al.2004). Removal of sodium azide is recommended prior to use in functional assays, the use of EQU003 is recommended for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD273 Antibody, clone TY25 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.J Immunol. 172 (4): 2530-41. 2. Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.J Immunol. 169 (10): 5538-45. 3. Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.J Immunol. 171 (8): 4156-63. 4. Furuhashi K et al. (2012) Mouse lung CD103+ and CD11b high dendritic cells preferentially induce distinct CD4+ T-cell responses.Am J Respir Cell Mol Biol. 46 (2): 165-72. 5. Yamazaki, T. et al. (2005) Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production.J Immunol. 175: 1586-92. 6. López-Medina M et al.(2015)Salmonella induces PD-L1 expression in B cells.Immunol Lett. pii: S0165-2478(15)30018-3. 7. López-Medina M et al.(2015)Salmonella impairs CD8 T cell response through PD-1: PD-L axis.Immunobiology. pii: S0171-2985(15)30028-0.
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