|Application ||WB, IF, E|
|Purification||Purified IgG prepared by affinity chromatography.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7M.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Phosphotyrosine Antibody, clone PY20 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Ruff-Jamison, S. et al. (1991) Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features.J. Biol. Chem. 266: 6607-6613. 2. Takagi, S. et al. (1991) Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells.J. Exp. Med. 174: 381-388. 3. Glenney, J.R. Jr. et al. (1988) Monoclonal antibodies to phosphotyrosine.J. Immunol. Methods. 109: 277-285. 4. Vendel,A.C. et al. (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.J Immunol. 182: 1509-17.
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