|Application ||IHC-P, IHC-F, FC|
|Calculated MW||26216 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3|
|Target/Specificity||Mouse anti-Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallonet al.1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype.Mouse anti-Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta(Xuet al.2012)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD16 Antibody, clone DJ130c is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Schmidt, R.E. (1993) CD16 cluster workshop report. In Leucocyte Typing V: White cell differentiation antigens, Vol.1. Edited by Schlossman, S.F. et al. Oxford University Press. p805 – 806. 3. Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.Diab Vasc Dis Res. 8: 221-8. 4. Shantsila, E. et al. (2012) Fibrinolytic status in acute coronary syndromes: evidence of differences in relation to clinical features and pathophysiological pathways.Thromb Haemost. 108: 32-40. 5. Shantsila, E. et al. (2011) Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.J Thromb Haemost. 9: 1056-66. 6. Tapp, L.D. et al. (2012) The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction.J Thromb Haemost. 10: 1231-41. 7. Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.Arthritis Res Ther. 14: R74. 8. Ambarus, C.A. et al. (2012) Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.J Immunol Methods. 375: 196-206. 9. Ambarus, C.A. et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.PLoS One. 7: e35994. 10. Shantsila, E. et al. (2012) The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates.Eur J Clin Invest. 42: 832-9. 11. Chehadeh. W. et al. (2009) Antibody-mediated opsonization of red blood cells in parvovirus B19 infection.Virology. 390: 56-63. 12. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.Circ Heart Fail. 6: 127-35. 13. Jaipersad, A.S. et al. (2014) Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis.Ann Med. 11: 1-9. 14. Shantsila, E. et al. (2015) Free Light Chains in patients with acute coronary syndromes: Relationships to inflammation and renal function.Int J Cardiol. 185: 322-7. 15. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.Circ Heart Fail. 6 (1): 127-35. 16. Romee R et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17).Blood. 121 (18): 3599-608.
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