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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig Granulocytes Antibody, clone 6D10    

Anti-Pig Granulocytes Antibody, clone 6D10 (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig Granulocytes Antibody, clone 6D10 (FITC) ABD12310
    Pig granulocytes stained with Mouse anti Pig granulocytes followed by Rabbit F(ab')2 anti Mouse IgG:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 6D10
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Porcine bone marrow haematopoietic cells (BMHC).
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig Granulocytes antibody, clone 6D10 recognizes a 60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (product code (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.Clone 6D10 has been shown as suitable for use on cytospins (Perezet al.2007).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig Granulocytes Antibody, clone 6D10 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Perez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
Dev. Comp. Immunol. 31: 296-306. 2. Stone JP et al. (2015) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.
Am J Transplant. Sep 14. [Epub ahead of print] 3. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
Dev Comp Immunol. 33 (3): 284-98.2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD12310
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