|Application ||IHC-F, FC, IP|
|Calculated MW||25415 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified human NK cells cultured in IL-2 (Bennettet al.1996)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A, Natural killer cell surface protein P1A, CD161, KLRB1, CLEC5B, NKRP1A|
|Target/Specificity||Mouse anti-Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanieret al.1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemiret al.2005).Mouse anti-Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanieret al.1994).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD161 Antibody, clone B199.2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bennett, I.M. et al. (1996) Definition of natural killer NKR-PIA+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. J. Exp. Med. 184: 1845-1856. 2. Azzoni, L. et al. (1998) Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells.J. Immunol. 161: 3493-3500. 3. de Lalla, C. et al. (2011) Invariant NKT Cell Reconstitution in Pediatric Leukemia Patients Given HLA-Haploidentical Stem Cell Transplantation Defines Distinct CD4+ and CD4- Subset Dynamics and Correlates with Remission State.J Immunol. 186: 4490-9. 4. Huarte, E. et al. (2008) PILAR is a novel modulator of human T-cell expansion.Blood. 112: 1259-68. 5. Williams, P.J. et al. (2009) Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy.Reproduction. 138: 177-84. 6. Higai, K. et al. (2006) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa protein.Biochim Biophys Acta. 1760: 1355-63. 7. Birchall, M.A. et al. (2008) Immunologic response of the laryngeal mucosa to extraesophageal reflux.Ann Otol Rhinol Laryngol. 117: 891-5. 8. Bossard, C. et al. (2012) Plasmacytoid dendritic cells and Th17 immune response contribution in gastrointestinal acute graft-versus-host disease.Leukemia. 26: 1471-4. 9. Higai, K. et al. (2008) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa proteinBiochim Biophys Acta. 1760: 1355-63. 10. Richter, J. et al. (2010) CD161 receptor participates in both impairing NK cell cytotoxicity and the response to glycans and vimentin in patients with rheumatoid arthritis.Clin Immunol. 136: 139-47. 11. Abrahamsson, S.V. et al. (2013) Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.Brain. 136: 2888-903. 12. Rother S et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.PLoS One. 10 (8): e0135682.
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