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>   home   >   Products   >   Primary Antibodies   >   Anti-Mouse CD68 Antibody, clone FA-11 (RPE)   

Anti-Mouse CD68 Antibody, clone FA-11

Rat Anti-Mouse Monoclonal Antibody

  • IHC - Anti-Mouse CD68 Antibody, clone FA-11  ABD12354
    Published customer image:Rat anti Mouse CD68 antibody, clone FA-11 used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded material.Image caption:Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P < 0.05 vs. UUO, ** P < 0.01 vs. UUO). Magnification x 400.From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1. PLoS ONE 10: e0116888.
  • IHC - Anti-Mouse CD68 Antibody, clone FA-11  ABD12354
    Published customer image:Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse brain by immunohistochemistry on cryostat sections.Image caption:Primed innate immune response in the brain following a systemic bacterial infection. Phenotypic changes after intracerebral injection of 100 pg of LPS in naïve mice (A, B, E, F, I, J, M, N) or mice pretreated with SL3261 (C, D, G, H, K, L, O, P). Representative images of immune marker expression (CD11c (A-D), CD68 (E-H), MHCII (I-L) in the injected hemisphere (B, D, F, H, J, L, N, P) or contralateral site are shown. Panels M-P show a double immunofluorescent stain for MHCII and laminin . n=5 animals per group, black scale bar=50μm, white scale bar=75μm.From: Püntener U, Booth SG, Perry VH, Teeling JL. Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.J Neuroinflammation. 2012 Jun 27;9:146.
  • IHC-P - Anti-Mouse CD68 Antibody, clone FA-11  ABD12354
    Published customer image:Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse lung and liver by immunohistochemistry on formalin fixed, paraffin embedded tissues.Image caption:Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections . The macrophages were indicated by anti-CD68 immunostaining . Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 μm for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody . Scale bar was 40 μm for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean ≤S.E. The p values were from Student&#39s t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9: e116023.
  • IHC - Anti-Mouse CD68 Antibody, clone FA-11  ABD12354
    Frozen mouse lymph node stained with rat anti-mouse CD68 at a 1/100 dilution followed by goat anti-rat IgG:HRP at a 1/50 dilution.
  • IHC - Anti-Mouse CD68 Antibody, clone FA-11  ABD12354
    Frozen mouse lymph node stained with rat anti-mouse CD68 at a 1/100 dilution followed by goat anti-rat IgG:HRP at a 1/50 dilution.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31996
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names FA-11
Calculated MW 34818 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Shelf Life 18 months from date of despatch.
Gene ID 12514
Other Names Macrosialin, CD68, Cd68
Target/Specificity Rat anti-mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.Rat anti-mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues (Masakiet al.2003) and Deveyet al.2009).Rat anti-mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD68 Antibody, clone FA-11 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Ramprasad, M.P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein.
Proc Natl Acad Sci U S A. 93 (25): 14833-8. 2. Rabinowitz, S.S. & Gordon, S. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli.
J Exp Med. 174 (4): 827-36. 3. da Silva, R.P. & Gordon, S. (1999) Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein.
Biochem J. 338 ( Pt 3): 687-94. 4. Schleicher, U. et al. (2005) Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-{gamma} in IL-12/IL-18-stimulated mouse macrophage populations.
Blood 105: 1319-1328. 5. Choi, E.J. et al. (2014) Novel brain arteriovenous malformation mouse models for type 1 hereditary hemorrhagic telangiectasia.
PLoS One. 9(2): e88511. 6. Kassim, S. et al. (2010) Gene therapy in a humanized mouse model of familial hypercholesterolemia leads to marked regression of atherosclerosis.
PloS ONE 5: e13424. 7. Rahaman, S.O. et al. (2011) Vav family Rho guanine nucleotide exchange factors regulate CD36-mediated macrophage foam cell formation.
J Biol Chem. 286: 7010-7. 8. Frossard, J.L. et al. (2011) Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury.
J Clin Pathol. 64: 387-93 9. West, E.L. et al. (2010) Long-term survival of photoreceptors transplanted into the adult murine neural retina requires immune modulation.
Stem Cells. 28: 1997-2007. 10. Lopez, M.E. et al. (2011) Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder.
J Neurosci. 31: 4367-78. 11. Jayagopal, A. et al. (2009) Quantum dot mediated imaging of atherosclerosis.
Nanotechnology. 20: 165102. 12. Leung, V.W. et al. (2009) Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.
Am J Pathol. 175: 1757-67. 13. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. 14. Lu, W. et al. (2010) Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres.
Biomaterials. 31: 2617-26. 15. de Beer, M.C. et al. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism.
J Lipid Res. 44: 674-85. 16. Song, L. et al. (2011) Deletion of the murine scavenger receptor CD68.
J Lipid Res. 52: 1542-50. 17. Daldrup-Link, H.E. et al. (2011) MR Imaging of Tumor Associated Macrophages with Clinically-Applicable Iron Oxide Nanoparticles.
Clin Cancer Res. 17: 5695-704. 18. Macauley, S.L. et al. (2011) The Role of Attenuated Astrocyte Activation in Infantile Neuronal Ceroid Lipofuscinosis
J. Neurosci 31: 15575-85. 19. Martin-Manso, G. et al. (2008) Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells.
Cancer Res. 68: 7090-9. 20. Lazarini, F. et al. (2012) Early Activation of Microglia Triggers Long-Lasting Impairment of Adult Neurogenesis in the Olfactory Bulb
J Neurosci 32: 3652-64 21. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
J Immunol. 182: 1278-86. 22. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
PLoS One. 7: e42654. 23. Smith, M. and Koch, G. (1987) Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.
J Cell Sci. 87:113-9. 24. Masaki, T. et al. (2003) Heterogeneity of antigen expression explains controversy over glomerular macrophage accumulation in mouse glomerulonephritis.
Nephrol. Dial. Transplant 18:178-81. 25. Dormishian, M. et al. (2013) Prokineticin receptor-1 is a new regulator of endothelial insulin uptake and capillary formation to control insulin sensitivity and cardiovascular and kidney functions.
J Am Heart Assoc. 2 (5): e000411. 26. von Bargen, K. et al. (2014) Cervical Lymph Nodes as a Selective Niche for Brucella during Oral Infections.
PLoS One. 10 (4): e0121790. 27. Hamour, S. et al. (2015) Local IL-17 Production Exerts a Protective Role in Murine Experimental Glomerulonephritis.
PLoS One. 10 (8): e0136238.

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