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Anti Rat CD11b Antibody, clone OX-42

Mouse Anti-Rat Monoclonal Antibody

  • FC - Anti Rat CD11b Antibody, clone OX-42  ABD12396
    Staining of rat peritoneal macrophages with Mouse anti Rat CD11b:RPE-Alexa Fluor® 647
  • FC - Anti Rat CD11b Antibody, clone OX-42  ABD12396
    Staining of rat peritoneal macrophages cells with Mouse anti Rat CD11b:Alexa Fluor® 488
  • FC - Anti Rat CD11b Antibody, clone OX-42  ABD12396
    Staining of rat peritoneal macrophages with Mouse anti Rat CD11b
  • FC - Anti Rat CD11b Antibody, clone OX-42  ABD12396
    Staining of rat peritoneal macrophages with Mouse anti Rat CD11b: Biotin
  • IHC - Anti Rat CD11b Antibody, clone OX-42  ABD12396
    Published customer image:Mouse anti Rat CD11b antibody, clone OX-42 used for Immunohistochemistry on cryostat sections.Image captionImmunohistochemical analysis of microglial activation and neuronal loss on day 3 post-treatment with UCN. Representative photomicrographs show: (A) low power images of peri-hematomal regions as indicated by squares; (B) high power images stained with OX-42; (C) ED-1; and (D) NeuN. (E) Average cell numbers (OX 42+, ED-1+, and NeuN+) in each plane (total 6 mm2) was calculated from 54 squares in 9 planes. Values are shown as mean ± SD, calculated and analyzed by Student's t test. *p < 0.05 vs. ICH + saline group.From: Liew HK, Pang CY, Hsu CW, Wang MJ, Li TY, Peng HF, Kuo JS, Wang JY. Systemic administration of urocortin after intracerebral hemorrhage reduces neurological deficits and neuroinflammation in rats. J Neuroinflammation. 2012 Jan 19;9:13. doi: 10.1186/1742-2094-9-13.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names OX-42
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Resident rat peritoneal macrophages.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single VWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinsonet al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinsonet al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD11b Antibody, clone OX-42 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12396
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