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Anti Human NQO1 Antibody, clone A180

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human NQO1 Antibody, clone A180  ABD12488
    Hela lysate probed with Mouse anti Human NQO1
  • WB - Anti Human NQO1 Antibody, clone A180  ABD12488
    Hela lysate probed with Mouse anti Human NQO1:HRP
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15559
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names A180
Calculated MW 30868 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Purified recombinant human NQO1
Shelf Life 18 months from date of despatch.
Gene ID 1728
Other Names NAD(P)H dehydrogenase [quinone] 1,, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, QR1, NQO1, DIA4, NMOR1
Target/Specificity Mouse anti human NQO1 antibody, clone A180 specifically recognises human NQO1 (NAD(P)H:quinone oxidoreductase 1), a 31kDa cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones. NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al.2002). Clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Sato, M. et al.2010andFegerholm, R. et al.2008).Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegal, D. et al.1998).Clone A180 is reported as suitable for use in immunofluorescence studies.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human NQO1 Antibody, clone A180 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12488
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