Anti Influenza A Matrix Protein Antibody, clone GA2B

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Published customer image:Mouse anti Influenza A Matrix Protein antibody, clone GA2B used for the evaluation of influenza matrix protein expression in infected cell lysates by western blotting.Image caption:IAV attachment induces EGFR endocytosis. (A) Upon treatment with sialidase (± sialidase; 0.01 units ml-1, 3 h, 37°C), A549 cells were infected with FPV (MOI = 100) or were stimulated with EGF, each for 1 h at 4°C and 30 min at 37°C. An EGFR-specific mouse antiserum and Alexa 488-conjugated chicken anti-mouse IgG as well as a HA-specific rabbit antiserum and an Alexa 594-conjugated chicken anti-rabbit IgG were employed. Cells were examined by IF microscopy. (B, C) A549 cells were infected with FPV (MOI = 100) or (B) incubated with EGF (30 ng ml-1) for 1 h at 4°C upon treatment with sialidase (± sialidase; 0.01 units ml-1, 3 h, 37°C). After a temperature shift cells were further kept at 37°C for the indicated times. (B) Surface resident EGFR was detected by FACS analysis. Fluorescence of uninfected/-stimulated cells was arbitrarily set as 100%. (C) After an acidic wash (PBS-HCl, pH 1.3), cells were permeabilized with saponin (0.2% w/v). Infected cells were assessed by detection of viral HA in FACS analysis. (D, E, F, G) A549 cells were transfected with a control or specific siRNAs targeting caveolin-1 (CAV-1) or clathrin heavy chain . (D) 48 h post transfection, knock-down was verified by WB using the indicated antibodies. (E, F) upon knock-down cells were (E) infected with FPV or (F) incubated with EGF as described in (B), without sialidase treatment. Cell surface EGFR was detected as mentioned in (B). (G) FPV internalization upon knock-down of the indicated proteins was analysed as described in (C).From: Eierhoff T, Hrincius ER, Rescher U, Ludwig S, Ehrhardt C The Epidermal Growth Factor Receptor Promotes Uptake of Influenza A Viruses into Host Cells. PLoS Pathog 6(9): e1001099.
Published customer image:Mouse anti Influenza A Matrix Protein antibody, clone GA2B used for the evaluation of influenza matrix protein expression in infected cell lysates by western blotting.Image caption:Tyrosine kinase activity is required for efficient uptake of IAV. (A, B, C) A549 cells were treated with genistein (50 μM, [+]) or (D, E) a mixture of several RTK inhibitors (each 10 μM, [+] see experimental procedures) or the solvent control (DMSO,[-]) respectively for 60 min at 37°C or over night prior to infection. (A) Upon genistein treatment, cells were either mock infected or infected with influenza virus A/FPV/Bratislava/79 (FPV; MOI?=?100) for further 30 min. Virus particles were visualized in immunofluorescence microscopy, via a hemagglutinin -specific rabbit antiserum and an Alexa 594-conjugated chicken anti-rabbit IgG. The nuclei were stained with DAPI. The white arrowheads point to virus particles lining at the cell border. (B, D) After infection with influenza virus A/Puerto-Rico/8/34 PR8 (MOI?=?8) for further 30 min, an acidic wash (PBS, pH 1.3, 4°C) was performed before cell-lysis. Internalized virus particles were visualized with a M1 monoclonal antibody in Western-blot analysis. Equal protein load was verified by ERK2 detection. The relative amount of M1 was quantified (B and D lower panels). Relative M1 densities are expressed as mean ±SD of three independent experiments. (C, E) Cells were infected with FPV or PR8 (MOI?=?4) for 8 h; upon the virus-attachment period an acidic wash (PBS-HCl, pH 5.5) was included. Progeny virus titers were determined by standard plaque assays. Data represent mean values of at least three independent experiments ±SD. Statistical significance was assessed by student's t-test (*) p
Published customer image:Mouse anti Influenza A Matrix Protein antibody, clone GA2B used for the evaluation of influenza matrix protein expression in infected cell lysates by western blotting.Image caption:EGFR knock-down impairs efficient uptake of IAV into A549 cells. (A) A549 cells were treated with an EGFR-specific antibody (5 μg ml-1) or mouse serum at the indicated times before and during infection with FPV (MOI = 0.5) for 8 h. Progeny virus titers were determined by standard plaque assays; virus yields of serum treated cells were arbitrarily set as 100%. Calculation of virus titers revealed 5.65×105 PFU ml-1(±1.2×105) for -30 min pre-incubation, 10×105 PFU ml-1(±1×105) for anti EGFR- and for serum pre-incubation. Statistical significance was assessed by student's t-test (*) p
Published customer image:Mouse anti Influenza A Matrix Protein antibody, clone GA2B used for the evaluation of influenza matrix protein expression in infected cell lysates by western blotting.Image caption:Attachment of IAV clusters plasma-membrane lipids. (A, B) A549 cells were infected with FPV (MOI = 100), stimulated with EGF (100 ng ml-1) or left untreated for 1 h at 4°C. Subsequently cells were incubated with the FITC-conjugated Choleratoxin beta subunit (30 μg ml-1) for 1 h at 4°C to visualize ganglioside M1 . (B) FPV was visualized by detection of HA via an HA-specific rabbit antiserum and an Alexa 594-conjugated chicken anti-rabbit IgG (right panel) or via an H7-HA-specific mouse antiserum and a Texas-Red conjugated goat anti-mouse IgG (middle panel). EGFR was detected by an EGFR-specific mouse antiserum followed by a Texas-Red conjugated goat anti-mouse IgG (left panel) or a Alexa 488-conjugated chicken anti-mouse IgG (right panel). For co-patching analysis CtxB was cross-linked by a rabbit antiserum against CtxB. Cells were examined by confocal laser scanning-microscopy. The colocalization was quantified as described in the experimental procedure section. (C, D, E) A549 cells were incubated with methyl-β-cyclodextrin (40 μg ml-1) for 1 h at 37°C and subsequently washed with PBS to withdraw MCD. (C) Cells were stained for GM1 with FITC-conjugated CtxB for 1 h at 4°C and subsequently incubated with rabbit antiserum against CtxB. (D) Cells were infected with PR8 (MOI = 4) for 1 h at 37°C; an acidic wash (PBS-HCl; pH 1.3, 4°C) was performed. In WB analysis M1 and ERK2 were detected. Relative M1 densities are expressed as mean values ±SD of at least three independent experiments. (E) Cells were infected with FPV or PR8 (MOI = 4); upon the attachment period an acidic wash (PBS-HCl, pH 5.5) was performed. Progeny virus titers were determined by plaque assays. Data represent mean values of three independent experiments ±SD. Statistical significance was assessed by student's t-test (*) p
Published customer image:Mouse anti Influenza A Matrix Protein antibody, clone GA2B used for the evaluation of influenza matrix protein expression in infected cell lysates by western blotting.Image caption:Characterization of influenza H5N1 VLPs. A) A schematic diagram of wild type and mutant H5 HA. The mutant H5 HA is showing a deletion of polybasic amino acids in the cleavage region of HA (?H5 HA). B) Western blot analysis of purified H5N1 VLPs. Lane 1, Influenza H5N1 VLPs. Lane 2, M1 VLPs lacking H5 HA. C) Cleavage of HA in VLPs. H5N1 VLPs were incubated without (-) or with (+) TPCK-treated trypsin (2.0 μg/ml trypsin), resolved on SDS-PAGE, and probed by western blot. D) Negative stain electron microscopy of influenza H5N1 VLPs.From: Kang S-M, Yoo D-G, Lipatov AS, Song J-M, Davis CT, Quan F-S, et al. Induction of Long-Term Protective Immune Responses by Influenza H5N1 Virus-Like Particles.PLoS ONE 4(3): e4667.

Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC-P, IF
Primary Accession	P03485
Other Accession	P03487
Reactivity	Virus
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone Names	GA2B
Calculated MW	27893 Da

Purification	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen	Influenza A / Puerto Rico / 8 / 34 (H1N1) and A/Bangkok / 1 / 79 (H3N2) viruses.
Shelf Life	18 months from date of despatch.
Gene ID	956527
Other Names	Matrix protein 1, M1, M
Target/Specificity	Mouse anti Influenza A matrix protein 1 antibody, clone GA2B recognizes an epitope within the influenza A matrix protein 1. In both strains of virus used as immunogen to isolate clone GA2B, the matrix protein 1 is a 252 amino acid, highly conserved viral protein playing a crucial role in replication. Mouse anti Influenza A matrix protein 1 antibody, clone GA2B can be used in influenza A IFA typing in conjunction with Mouse anti Influenza A matrix protein, clone AA5H.
Preservative & Stabilisers	0.09%Sodium Azide
Storage	Store at +4℃ or at -20℃ if preferred.
Precautions	Anti Influenza A Matrix Protein Antibody, clone GA2B is for research use only and not for use in diagnostic or therapeutic procedures.

Mouse Anti-Viral Monoclonal Antibody

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