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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti Human Cytochrome B245 Heavy Chain Antibody, clone NL7    

Anti Human Cytochrome B245 Heavy Chain Antibody, clone NL7

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti Human Cytochrome B245 Heavy Chain Antibody, clone NL7  ABD12593
    Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain . Permeabilised with Leucoperm
  • IF - Anti Human Cytochrome B245 Heavy Chain Antibody, clone NL7  ABD12593
    Published customer image:Mouse anti Human Cytochrome B245 Heavy Chain antibody, clone NL7 used for the identification of gp91PHOX expressing cells in mouse brain by immunofluorescence.Image caption:Effect of a-SYN over-expression on microglial activation in wild-type and Fc?R-/- mice. A-D) At four weeks after AAV injection, SN sections of wild-type and Fc?R-/- mice over-expressing a-SYN or GFP were double-stained for gp91PHOX and SYN/GFP . Wild-type mice expressing a-SYN revealed significantly increased immunoreactivity for gp91PHOX compared to the other three groups. gp91PHOX immunoreactivity was localized to microglia, which appear to be close to a-SYN-expressing neurons (scale bar = 50 µm). E) Scoring of microglial activation using a rating scale revealed significant microglial activation in wild-type mice expressing a-SYN compared to those expressing GFP. This difference was not observed in Fc?R-/- mice expressing SYN or GFP. *p < 0.05, WT-SYN vs WT-GFP, Mann-Whitney U test.From: Cao S, Theodore S, Standaert DG. Fc? receptors are required for NF-?B signaling, microglial activation and dopaminergic neurodegeneration in an AAV-synuclein mouse model of Parkinson's disease.Mol Neurodegener. 2010 Oct 26;5:42.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04839
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names NL7
Calculated MW 65336 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Cytochrome B245 solubilised in octyl-beta-glucopyranoside.
Shelf Life 18 months from date of despatch.
Gene ID 1536
Other Names Cytochrome b-245 heavy chain, 1.-.-.-, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, CYBB, NOX2
Target/Specificity Mouse anti Human Cytochrome B245 Heavy Chain antibody, clone NL7 recognizes the heavy chain of cytochrome B245, also known as gp91phox or NOX2, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. Mouse anti Human Cytochrome B245 Heavy Chain antibody, clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils. It recognizes the native as well as the denatured form of the protein. Clone NL7 has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Cytochrome B245 Heavy Chain Antibody, clone NL7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12593
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