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Anti Human CD54 Antibody, clone 84H10

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human CD54 Antibody, clone 84H10  ABD12682
    Published customer image:ICAM-1 activates PKCa but not PKCßII in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCßII and PKCa was examined by western blot with B) anti-phospho-PKCßIIThr641 or anti-PKCßII or C–D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean ± SEM of 3 experiments. NT, nontreated. *, p
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC, E, IP, Func
Primary Accession P05362
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 84H10
Calculated MW 57825 Da
Additional Information
Other Species Dog
Purification Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen K562 cell line
Shelf Life 18 months from date of despatch.
Gene ID 3383
Other Names Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Target/Specificity Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD.Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD54 Antibody, clone 84H10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12682
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