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Anti Chicken CD25 Antibody, clone AV142

Mouse Anti-Chicken Monoclonal Antibody

  • E - Anti Chicken CD25 Antibody, clone AV142  ABD12807
    ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody . Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.)
  • WB - Anti Chicken CD25 Antibody, clone AV142  ABD12807
    Western blot analysis of chicken CD25/huIg fusion protein with Mouse anti Chicken CD25 . Recombinant huIg was used as negative control (Image courtesy of the Roslin Institute.)
  • FC - Anti Chicken CD25 Antibody, clone AV142  ABD12807
    Staining of ConA-stimulated chicken splenocytes with Mouse anti Chicken CD25 conjugated with Alexa fluor-647(Image courtesy of the Roslin Institute.)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Chicken
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names AV142
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Chicken CD25/huIg fusion protein.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Chicken CD25 monoclonal antibody, clone AV142 recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Rα) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature. Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes. CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection . Chicken IL-2Rα is the low affinity receptor for Interleukin-2.Mouse anti Chicken CD25 monoclonal antibody, clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from AbD Serotec.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Chicken CD25 Antibody, clone AV142 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12807
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