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Anti Human CD14 Antibody, clone UCHM1

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human CD14 Antibody, clone UCHM1  ABD12821
    Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively
  • FC - Anti Human CD14 Antibody, clone UCHM1  ABD12821
    Figure A. FITC conjugated mouse anti human CD66b and APC conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD66b and APC conjugated mouse anti human CD14 . All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock .
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08571
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names UCHM1
Calculated MW 40076 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Human Thymocytes followed by peripheral blood mononuclear cells.
Shelf Life 18 months from date of despatch.
Gene ID 929
Other Names Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Target/Specificity Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of 55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative  (Hogget al.1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3),Sucrose
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti Human CD14 Antibody, clone UCHM1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12821
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