My Cart 0
Register or Login
(888) 735 - 7227
CONTACT US
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4    

Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4

Rat Anti-Mouse Monoclonal Antibody

     
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.From: Tian Y, Linden J, French BA, Yang Z Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9: e114375.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Renal inflammatory changes in Ntn1+/- mice following ischemia. Ntn1+/- mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A–D) Neutrophil staining. Arrows indicate neutrophils (magnification 400×). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase . (F) TNF-a and (G) interleukin-6 (IL-6) and (H) interleukin-10 were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to ß-actin and are expressed as fold change compared to sham-operated animals without ischemia (-I). Data are representative of four to six independent experiments for each experimental condition (mean ± SD).From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 µm in length. All micrographs were taken using 200× magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.
  • FC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the identification of infiltrating neutrophils in murine pulmonary tissue sections by immunohistochemistry.Image caption:Apigenin decreases LPS-induced neutrophil infiltration in the lungs. Lungs from mice receiving Api + LPS, LPS, Api or vehicle were collected at 24 h post-challenge. (A) H&E staining; (B) Chloroacetate esterase staining; (C) Lungs stained with anti-7/4 antibodies; (D) Leukocyte infiltration determined by counting CAE-positive cells per high power field in lungs used in (B); (E) Number of infiltrated neutrophils as determined by counting stained cells per cpf in in lungs used in (C). Data represent the mean ± SEM (N = 9 mice for each biological condition; * p < 0.05 vs. LPS). Scale bars for all figures are 25 μm and 100 μm for insets.From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.Int. J. Mol. Sci. 2016, 17, 323.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the detection of neutrophils in murine kidney tissue sections by immunohistochemistry. Macrophages were detected using Rat anti Mouse F4/80 antibody, clone A3-1 Image caption:Immunostaining of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.From: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.PLoS ONE 11(3): e0150886.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 7/4
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Cultured bone marrow cells
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosaset al.2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosaset al.2010).In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimuraet al.1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncelloet al.1991) and the depletion of myeloid cells in vivo(Rosaset al.2010).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol
Advance Filter
FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Submit Review
Cat# ABD12869
Availability: Inquire
INQUIRE
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
orders@abgent.com
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900
sales@abgent.com
orders@abgent.com
tech@abgent.com
fn@abgent.com

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms and Conditions
Terms & Conditions
"
SERVICES
ELITE Custom Services
Antibody Services
Peptide Synthesis
Protein Expression
Validation Services
Citations
PRODUCTS
Crown Products
New Products
Primary Antibodies
Secondary Antibodies
Cell/Tissues/Lysates
FLcDNA Clones
All Products
Citations
ORDERS/CONTACT
USA Orders
International Orders
COMPANY
About Us
Company Profile
Business Development
News & Announcements
Contact
SUPPORT
Technical Support
Useful Links
Custom Service Tracking
Literature
Promotions
Feedback
Tools
Copyright © 2019 Abgent. All Rights Reserved
sitemap  |  privacy  |  terms and conditions