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Anti Mouse CD169 Antibody, clone MOMA-1

Rat Anti-Mouse Monoclonal Antibody

  • IF - Anti Mouse CD169 Antibody, clone MOMA-1  ABD12923
    Published customer image:FITC conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used for the detection of marginal zone macrophages in mouse spleen by immunofluorescence of formalin fixed tissue cryosectionsImage caption:Common hematopoietic markers in lymphoid organs do not correlate with prion deposition. Frozen sections from spleens (A, C, E & G) and mesenteric lymph nodes (B, D, F & H) from C57BL/6 Ig-treated, C57BL/6 LTßR-Ig-treated, TNFR1-/- Ig-treated, or TNFR1-/- LTßR-Ig-treated mice were analyzed by immunohistochemistry and developed with alkaline phosphatase (A–D) or immunofluorescence (E–H) for macrophages (F4/80; A & B), B-cells (B-cells; C & D), metallophilic macrophages (MOMA-1; E & F), and T-cells (CD3; G & H). Size bars in A & C = 100 µm; B & D = 200 µm; E–H = 100 µm.From: O'Connor T, Frei N, Sponarova J, Schwarz P, Heikenwalder M, Aguzzi A Lymphotoxin, but Not TNF, Is Required for Prion Invasion of Lymph Nodes.PLoS Pathogens 8(8): e1002867.
  • IF - Anti Mouse CD169 Antibody, clone MOMA-1  ABD12923
    Published customer image:FITC-conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used for the identification of marginal zone metalophilic macrophagesin murine spleen by immunofluorescence on formalin fixed cryosections. Image caption:IgM from BALB/c mice enhances germinal center responses. On day 0, BALB/c mice were immunized i.v. with WT or C µ13 IgM specific for KLH (50 µg/mouse) or SRBC (0.2 ml of a solution with HA titer 1:32) 30 min before 10 µg KLH (A), 5×105 (B) or 5×106 SRBC (C) were administered via the same route; controls received antigens or specific IgM alone. Spleens were harvested on day 10. Splenocytes from half of each spleen were analyzed by flow cytometry; germinal center B cells were gated as GL7+PNA+ amongst B220+ cells (Figure S1) and the percentages of germinal center B cells were quantified (A-C, upper left panels). The other halves of the spleens were sectioned, stained with anti-B220 , anti-MOMA and PNA , and analyzed for number of PNA+ germinal centers in B cell follicles by confocal microscopy (A-C, upper right panels); each image is a representative area (1725 µm × 1295 µm) for 2-3 whole sections with original magnification ×10 (A-C, lower panels). Germinal center responses of mice immunized with specific IgM alone were always lower than the responses of mice immunized with antigens alone (not shown). Data are representative of two experiments with each antigen dose. ns = not significant; * = p < 0.05; ** = p < 0.01.From: Ding Z, Bergman A, Rutemark C, Ouchida R, Ohno H, Wang J-Y, et al. Complement-Activating IgM Enhances the Humoral but Not the T Cell Immune Response in Mice.PLoS ONE 8: e81299.
  • IF - Anti Mouse CD169 Antibody, clone MOMA-1  ABD12923
    Published customer image:FITC-conjugated Rat anti Mouse CD169 antibody used to demonstrate marginal zone metalophilic macrophages in murine spleen by immunofluorescence on acetone fixed cryosections.Image caption:Plain PPS-1 booster s.c. abrogates the Pnc1-TT-induced GC reaction in mice primed as neonates. Active germinal centers in spleen sections were enumerated with PNA staining (upper panels). Double fluorescent staining was performed with PNA and MOMA-1 (metallophilic marginal macrophages) to show the follicular structure (top panel). IgM+ and IgG+ follicles were identified with anti-IgM (middle panel) and anti-IgG (lower panel) staining, 7 days after booster with 5.0 µg PPS-1 and 5.0 µg LT-K63 s.c. or i.n. . Spleen sections, 7 µm, were prepared from four different levels in the spleen, starting 700 µm into the tissue and each level separated by 210 µm. One representative section per group is shown. Results are from one representative of two independent experiments (8 mice per group) showing comparable results.From: Bjarnarson SP, Benonisson H, Del Giudice G, Jonsdottir I Pneumococcal Polysaccharide Abrogates Conjugate-Induced Germinal Center Reaction and Depletes Antibody Secreting Cell Pool, Causing Hyporesponsiveness.PLoS ONE 8(9): e72588.
  • IF - Anti Mouse CD169 Antibody, clone MOMA-1  ABD12923
    Published customer image:AlexaFluor 647&reg; Rat anti Mouse CD169 antibody, clone MOMA-1 used for the detection of marginal zone metallophils in mouse spleen by immunofluorescence.Image caption:A fraction of BEC is CDH17+. (A) Spleens were treated with dispase and collagenase IV to obtain single stromal cells, and cell surface markers were analyzed by flow cytometry. Stromal cells were separated into four subpopulations: fibroblastic reticular cells (FRCs, gp38+CD31-), lymphatic endothelial cells (LEC, gp38+CD31+), BEC (gp38-CD31+), and double-negative cells (far right panel). Each fraction was then tested for CDH17 expression. The numbers adjacent to the gates indicate the percentage (%) of the indicated cells within their respective parental gates (shown on top of each panel). (B) The percentages of CDH17+ stromal cells within the respective parental gates are plotted on a bar graph. The percentages were calculated by subtracting the values of KO mice from those of WT mice (n = 7 (MAdCAM-1+ BEC); n = 8 ). *P=0.05 (Student’s t-test). (C) WT mouse spleen was stained with anti-CD169 , anti-MAdCAM-1 , anti-mouse IgG1 , anti-CDH17 (red, BD1B), and analyzed by confocal microscopy. The middle-right micrograph is an enlarged view of the boxed area shown in the middle-left micrograph. The bottom row of images shows each separate color channel and an expanded view of the merged image shown within the box in the middle-right panel. The white arrowhead shown in the bottom right image indicates an IgG1+ cell that is adjacent to a CDH17+ cell in the MZ sinus. Data are representative of three independent experiments. Scale bars, 50 µm (top and middle row of images) or 10 µm (bottom row). (D) The localization of IgG1+ cells in the MZ, red pulp, and white pulp in WT and KO mice are plotted on a bar graph. Values are expressed as the percentage of IgG1+ cells localized within each sub-region of the spleen (n = 6; *P=0.05 (Student’s t-test)). (E) The number of MZ sinus (MAdCAM-1+) cells, CDH17+ cells, and CDH17+ MZ sinus cells (Cdh17+MAdCAM-1+) in the spleen of WT mice are plotted on a bar graph. Cell numbers were counted in six histological sections of WT spleen. The mean and standard deviation are plotted.From: Funakoshi S, Shimizu T, Numata O, Ato M, Melchers F, Ohnishi K BILL-Cadherin/Cadherin-17 Contributes to the Survival of Memory B Cells.PLoS ONE 10(1): e0117566.
  • IF - Anti Mouse CD169 Antibody, clone MOMA-1  ABD12923
    Published customer image:FITC conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used for the detection of CD169 expressing cells in murine spleen by immunofluorescence.Image caption:Marginal zone B cell regions were narrowed in Ziz2 KO mice. (A) Spleen sections were stained with anti-B220 and anti-CD169 antibodies for the MZ B cell region (B220-positive region outside CD169-positive cells). All mice were 10 weeks old. Scale bars: 100 µm. (B) MZ B cell regions were narrower in Ziz2 and Ziz3 KO mice than in wild type mice. (C) The proliferative activity of MZ B cells in response to LPS was not altered in both KO mice. Three mice per group were used. Data from three independent experiments (one mouse per group per experiment was used) were summarized. (D) The migratory activity of MZ B cells was analyzed using a transwell and flow cytometry. Activity against BLC or SDF1 was not altered in both KO mice. Four mice per group were used. Each plot indicates data from one mouse. Data from four independent experiments (one mouse per group per experiment was used) were summarized. 2KO: Ziz2 KO. 3KO: Ziz3 KO. ***: P?
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q62230
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names MOMA-1
Calculated MW 182979 Da
Additional Information
Purification Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serumPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue cul
Immunogen Stromal (reticular) elements from spleen.
Shelf Life 18 months from the date of despatch
Gene ID 20612
Other Names Sialoadhesin, Sheep erythrocyte receptor, SER, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, Siglec1, Sa, Sn
Target/Specificity Rat anti Mouse CD169, clone MOMA-1 recognizes murine CD169, also known as sialoadhesin or Siglec-1. CD169 is a lectin-like receptor expressed by certain populations of macrophages including marginal zone metallophils of the spleen, subcapsular macrophages of lymph nodes and stromal macrophages in bone marrow (Morriset al.1991).CD169 is a ~185 kDa sialic acid binding receptor containing 17 immunoglobulin-like domains (Crockeret al.1992). Expression of CD169 can be induced on macrophages in culture by a serum factor and further modulated by cytokine exposure (McWilliamet al.1992).Rat anti mouse CD169, clone MOMA-1 has been used for the in vivo depletion of specific macrophage populations (Kraalet al.1988).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse CD169 Antibody, clone MOMA-1 is for research use only and not for use in diagnostic or therapeutic procedures.
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