|Application ||WB, IP|
|Calculated MW||38868 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Bacterially produced recombinant full length human EBP50.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, EBP50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, SLC9A3R1, NHERF, NHERF1|
|Target/Specificity||Mouse anti Human EPB50 antibody, clone EPB-10 recognizes ezrin/radixin/moesin (ERM)-binding phosphoprotein of 50kDa (EBP50) otherwise known as Na+/H+ exchanger regulatory factor 1 (NHERF1), Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1 or Solute carrier family 9 isoform A3 regulatory factor 1. EBP50 is a 358 amino acid ~50kDa cytoplasmic adaptor molecule containing 2 PDZ domains. EBP50 is expressed in tissues containing polarized epithelia, including the small intestine, placenta, liver and kidney.The ERM family of membrane-cytoskeletal linking proteins act to maintain a regulated linkage between actin filaments (F-actin) and the plasma membrane. Studies have revealed that EBP50 is an important ERM binding partner, which interacts with the N-terminal FERM (four point one ERM) domain of ERM proteins, via its C-terminal domain. EBP50 colocalizes with actin and ezrin, in the apical mircovilli of epithelial cells and this role as binding partner has been further shown through the interaction of EBP50 with the transmembrane adaptor protein known as Protein Associated with Glycosphingolipid-enriched microdomains (PAG), in T cells (Reczeket al.1997). Mutations in the gene encoding EBP50 lead to the disease Nephrolithiasis/osteoporosis, hypophosphatemic, 2 characterized by impairedphosphate metabolism (Courbebaisseet al.2012). Other ligands which bind to the PDZ domains of EBP50, include beta-catenin, cystic fibrosis transmembrane conductance regulator and the type II Na/Pi cotransporter Npt2, implicating EBP50 in a diverse range of biological systems.Mouse anti Human EPB50 antibody, clone EPB-10 originally raised by Brdičková et al. has been sucessfully used in western blottingandimmunofluorescence studies of EBP expression and localization.|
|Preservative & Stabilisers||0.09%Sodium Azide|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human EBP50 Antibody, clone EBP-10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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