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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human EBP50 Antibody, clone EBP-10    

Anti Human EBP50 Antibody, clone EBP-10

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14745
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names EBP-10
Calculated MW 38868 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Bacterially produced recombinant full length human EBP50.
Shelf Life 18 months from date of despatch.
Gene ID 9368
Other Names Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, EBP50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, SLC9A3R1, NHERF, NHERF1
Target/Specificity Mouse anti Human EPB50 antibody, clone EPB-10 recognizes ezrin/radixin/moesin (ERM)-binding phosphoprotein of 50kDa (EBP50) otherwise known as Na+/H+ exchanger regulatory factor 1 (NHERF1), Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1 or Solute carrier family 9 isoform A3 regulatory factor 1. EBP50 is a 358 amino acid ~50kDa cytoplasmic adaptor molecule containing 2 PDZ domains. EBP50 is expressed in tissues containing polarized epithelia, including the small intestine, placenta, liver and kidney.The ERM family of membrane-cytoskeletal linking proteins act to maintain a regulated linkage between actin filaments (F-actin) and the plasma membrane. Studies have revealed that EBP50 is an important ERM binding partner, which interacts with the N-terminal FERM (four point one ERM) domain of ERM proteins, via its C-terminal domain. EBP50 colocalizes with actin and ezrin, in the apical mircovilli of epithelial cells and this role as binding partner has been further shown through the interaction of EBP50 with the transmembrane adaptor protein known as Protein Associated with Glycosphingolipid-enriched microdomains (PAG), in T cells (Reczeket al.1997). Mutations in the gene encoding EBP50 lead to the disease Nephrolithiasis/osteoporosis, hypophosphatemic, 2 characterized by impairedphosphate metabolism (Courbebaisseet al.2012). Other ligands which bind to the PDZ domains of EBP50, include beta-catenin, cystic fibrosis transmembrane conductance regulator and the type II Na/Pi cotransporter Npt2, implicating EBP50 in a diverse range of biological systems.Mouse anti Human EPB50 antibody, clone EPB-10 originally raised by Brdičková et al. has been sucessfully used in western blottingandimmunofluorescence studies of EBP expression and localization.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human EBP50 Antibody, clone EBP-10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13422
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