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Anti Mouse Kappa Light Chain Antibody, clone OX-20

  • E - Anti Mouse Kappa Light Chain Antibody, clone OX-20  ABD14083
    Purified Mouse IgG (PMP01) used as coating reagent in a direct ELISA detected with Rat anti Mouse Kappa Light Chain:HRP (ABD14083)
  • WB - Anti Mouse Kappa Light Chain Antibody, clone OX-20  ABD14083
    A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (ABD14083) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01837
Reactivity Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names OX-20
Calculated MW 11934 Da
Additional Information
Immunogen Purified mouse IgG.
Shelf Life 18 months from date of despatch.18 months from date of despatch.
Other Names Ig kappa chain C region, IGKC
Target/Specificity Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.
Storage Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC for one month or a
PrecautionsAnti Mouse Kappa Light Chain Antibody, clone OX-20 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Galfre, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG
Nature 277: 131-133.

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Cat# ABD14083
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