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Recombinant Human Interleukin-2

     
  • E - Recombinant Human Interleukin-2  ABD14324
    Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 (MCA1553) as a capture reagent and biotinylated Rabbit anti Human IL-2 (AHP1032B) as a detection reagent with recombinant human IL-2 (ABD14324) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the ment, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P60568
Reactivity Human
Calculated MW 17628 Da
Additional Information
Shelf Life 3 months from date of reconstitution.3 months from date of reconstitution.
Gene ID 3558
Other Names Interleukin-2, IL-2, T-cell growth factor, TCGF, Aldesleukin, IL2
Target/Specificity Recombinant human interleukin 2 is an Escherichia coli-derived recombinant protein corresponding to full-length mature human interleukin-2, a secreted peptide required for T-cell proliferation. This preparation has an ED50 of 0.1ng/ml as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.
Storage Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this pro
PrecautionsRecombinant Human Interleukin-2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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References

1. Akari, H. et al. (2000) Nef-induced major histocompatibility complex class I down-regulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation.
J. Virol. 74 (6): 2907 - 2912. 2. Chan, P.K. et al. (2010) T-cell response to human papillomavirus type 58 L1, E6 and E7 peptides in women with cleared infection, cervical intraepithelial neoplasia and invasive cancer.
Clin Vaccine Immunol. 17: 1315-21. 3. Doi, N. et al. (2010) Growth ability in various macaque cell lines of HIV-1 with simian cell-tropism.
J Med Invest. 57: 284-92. 4. Vaughan, K. et al. (2007) A DNA vaccine against dolphin morbillivirus is immunogenic in bottlenose dolphins.
Vet Immunol Immunopathol. 120: 260-6. 5. Ban, S.A. et al. (2014) Combined immunodeficiency evolving into predominant CD4+ lymphopenia caused by somatic chimerism in JAK3.
J Clin Immunol. 34 (8): 941-53. 6. Geoghegan, V. et al. (2015) Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling.
Nat Commun. 6: 6758. 7. Yutani, S. et al. (2017) Feasibility study of personalized peptide vaccination for hepatocellular carcinoma patients refractory to locoregional therapies.
Cancer Sci. 108 (9): 1732-8.

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Cat# ABD14324
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