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Recombinant Human Interleukin-7

     
  • E - Recombinant Human Interleukin-7  ABD14327
    Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 (1012201) as a capture reagent and biotinylated Rat anti Human IL-7 (1012308) as a detection reagent with recombinant human IL-7 (ABD14327) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A human serum sample is indicated (green)
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P13232
Reactivity Human
Calculated MW 20187 Da
Additional Information
Shelf Life 3 months from date of reconstitution.
Gene ID 3574
Other Names Interleukin-7, IL-7, IL7
Target/Specificity Recombinant human IL-7 is a 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence.IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106units/mg.Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clones BVD10-40F6andBVD10-11C10 in ELISA.
Storage Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this pro
PrecautionsRecombinant Human Interleukin-7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD14327
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