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Recombinant Yellow Fever Virus NS1 Protein

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Additional Information
Shelf Life 12 months from date of despatch.
Target/Specificity Recombinant yellow fever virus NS1 protein is produced as a hexamer in the human 293 cell line. This product has been purified and retains its native folding state and post-translational modifications providing optimal antigenicity. Research indicates that the hexamer is the biologically active form of the NS1 antigen and therefore involved in the pathogenesis of yellow fever virus (YFV).Yellow fever virus belongs to the Flaviviridae family and like closely related viruses, such as West Nile virus, is an abovirus utilizing various mosquito species as vectors. YFV appears to be restricted naturally to human and other primates but can be experimentally induced in other mammalian species. In recent years an increasing number cases of YFV infection have been reported.Originating in Africa, YFV has been introduced to tropical regions of South America and periodic outbreaks have historically occurred in both Europe and North America. According to the World Health Organization (WHO), it has been estimated that approximately 200,000 people contract YFV annually with around 30,000 cases resulting in fatality, the majority of these on the African continent.This product may be used in research to investigate vaccine development (Bonaldo, M.C. et al.2014) or in research into the development of assays to detect YFV infection (Ding, X.X,et al.2014).
Storage Store at -70oC.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation befo
PrecautionsRecombinant Yellow Fever Virus NS1 Protein is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Ding, X.X. et al. (2014) Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1.
PLoS One 9(10): e108623.2. Bonaldo, M.C. et al. (2014) The yellow fever 17D virus as a platform for new live attenuated vaccines.
Hum Vaccin Immunother. 10(5): 1256-65.

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Cat# ABD14462
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