|Calculated MW||27077 Da|
|Shelf Life||3 months from date of reconstitution.|
|Other Names||Beta-nerve growth factor, Beta-NGF, Ngf, Ngfb|
|Target/Specificity||Purified Mouse Nerve Growth Factor 2.5S is prepared form mouse submaxillary glands (Bocchini and Angeletti 1969) and has an apparent molecular mass of ~30 kDa. Nerve growth factor has a variety of effects on the growth and development of sensory and sympathetic neurons. In the peripheral nervous system, NGF is required for the development and maintenance of sympathetic nerve cells that use catecholamine neurotransmitters.Purified Mouse Nerve Growth Factor 2.5S has been used to demonstrate the importance of NGF in regulation of neuronal function through the up-regulation of the transcription factor NFAT (Nuclear Factor of Activated T-cells) via activation of the PI3K/Akt pathway (Kimet al.2014).|
|Storage||Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this pro|
|Precautions||Purified Mouse Nerve Growth Factor 2.5S is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Rohn, T.A. et al. (2011) A Virus-Like Particle-Based Anti-Nerve Growth Factor Vaccine Reduces Inflammatory Hyperalgesia: Potential Long-Term Therapy for Chronic Pain.J Immunol. 186: 1769-80. 2. Laursen, L.S. et al. (2011) Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP-K.J Cell Biol. 192: 797-811. 3. Colbert, R.A. et al. (1994) Vasoactive intestinal peptide stimulates neuropeptide Y gene expression and causes neurite extension in PC12 cells through independent mechanisms.J Neurosci. 14: 7141-7. 4. Smith-Thomas, L.C. et al. (1995) Increased axon regeneration in astrocytes grown in the presence of proteoglycan synthesis inhibitors.J Cell Sci. 108: 1307-15. 5. Barrie, A.P. et al. (1997) Pituitary adenylyl cyclase-activating peptide stimulates extracellular signal-regulated kinase 1 or 2 (ERK1/2) activity in a Ras-independent, mitogen-activated protein Kinase/ERK kinase 1 or 2-dependent manner in PC12 cells.J Biol Chem. 272: 19666-71. 6. Liu, N. et al. (2005) Enhancement of Schwann cell myelin formation by K252a in the Trembler-J mouse dorsal root ganglion explant culture.J Neurosci Res. 79: 310-7. 7. Eibl, J.K. et al. (2010) Multipotent neurotrophin antagonist targets brain-derived neurotrophic factor and nerve growth factor.J Pharmacol Exp Ther. 332: 446-54. 8. Vogelezang, M. et al. (2007) Neurite outgrowth on a fibronectin isoform expressed during peripheral nerve regeneration is mediated by the interaction of paxillin with alpha4beta1 integrins.BMC Neurosci. 8: 44. 9. Seiberlich, V. et al. (2015) Downregulation of the microtubule associated protein Tau impairs process outgrowth and myelin basic protein mRNA transport in oligodendrocytes.Glia. 63 (9): 1621-35. 10. von Büdingen, H.C. et al. (2015) The myelin oligodendrocyte glycoprotein directly binds nerve growth factor to modulate central axon circuitry.J Cell Biol. 210 (6): 891-8. 11. Miyamoto, Y. et al. (2015) Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination.Mol Biol Cell. 26 (19): 3489-503.1. Bocchini V & Angeletti PU (1969) The nerve growth factor: purification as a 30,000-molecular-weight protein.Proc Natl Acad Sci U S A. 64 (2): 787-94.
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