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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti Mouse IgG (Fc) Antibody    

Anti Mouse IgG (Fc) Antibody

     
  • WB - Anti Mouse IgG (Fc) Antibody  ABD14560
    A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (ABD14560P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession P01863
Other Accession P01865, P03987, P01867, P01868, P01864, P01869
Reactivity Mouse
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36389 Da
Additional Information
Purification Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Shelf Life 12 months from date of despatch.18 months from date of despatch.12 months from date of despatch.
Other Names Ig gamma-2A chain C region, A allele, Immunoglobulin heavy chain gamma polypeptide, Ighg
Target/Specificity Goat anti mouse IgG (Fc) polyclonal antibody reacts with mouse IgG at an epitope localised to the Fc region as assessed by immunoelectrophoresis and ELISA. Cross reactivity with IgA and IgM is negligible.Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.
Storage Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE.Thi
PrecautionsAnti Mouse IgG (Fc) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
Parasitology. 136: 691-7. 2. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58. 3. Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
PLoS One. 6(1):e15861. 4. Wegmann F et al. (2015) The carbomer-lecithin adjuvant Adjuplex™ has potent immune activating properties and elicits protective adaptive immunity against influenza challenge in mice.
Clin Vaccine Immunol. pii: CVI.00736-14. 5. Liu, Z. et al. (2015) Partial protective immunity against toxoplasmosis in mice elicited by recombinant Toxoplasma gondii malate dehydrogenase
Vaccine Oct 27 [Epub ahead of print] 6. Swaffer, M.P. et al. (2016) CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Cell. 167 (7): 1750-1761.e16. 7. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. Oct 08 [Epub ahead of print].

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Cat# ABD14560
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