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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti Human IgE Antibody    

Anti Human IgE Antibody

     
  • E - Anti Human IgE Antibody  ABD14583
    Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (ABD14583B) as a detection reagent with purified human IgE (Bio-Rad Catalog 55252300) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively
  • E - Anti Human IgE Antibody  ABD14583
    Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (ABD14583B) as a detection reagent with recombinant human IgE (HCA171) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively
  • E - Anti Human IgE Antibody  ABD14583
    Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (ABD14583B) as a detection reagent with recombinant human IgE (HCA190) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P01854
Reactivity Human
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 47019 Da
Additional Information
Purification Antisera to human IgE were raised by repeated immunisation of goats with highly purified antigen.
Immunogen Native human IgE.
Shelf Life 12 months from date of despatch.18 months from date of despatch.12 months from date of despatch.18 months from date of despatch.12 months from date of despatch.
Other Names Ig epsilon chain C region, IGHE
Target/Specificity Goat anti Human IgE polyclonal antibody specifically recognizes human IgE. Minimal cross-reactivity (
Storage Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitiv
PrecautionsAnti Human IgE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.
Allergy Asthma Clin Immunol. 8 (1): 1. 2. Pinot de Moira, A. et al. (2013) Effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection in childhood schistosome and hookworm coinfections.
Infect Immun. 81 (1): 23-32. 3. Zheng W et al. (2016) Upregulated expression of substance P in basophils of the patients with chronic spontaneous urticaria: induction of histamine release and basophil accumulation by substance P.
Cell Biol Toxicol. May 4. [Epub ahead of print] 4. Jin Y et al. (2015) Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure.
Food Chem Toxicol. 76: 86-93.

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Cat# ABD14583
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