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>   home   >   Products   >   Primary Antibodies   >   Goat anti Mouse IgG Antibody (H/L) - HRP    

Goat anti Mouse IgG Antibody (H/L) - HRP

  • WB - Goat anti Mouse IgG Antibody (H/L) - HRP  ABD14616
    A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (ABD14616) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Mouse
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Purification Antiserum to mouse IgG (H/L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.using antigens coupled to agarose beads.
Shelf Life 12 months from date of reconstitution.
Target/Specificity Goat anti Mouse IgG (H/L) polyclonal antibody reacts with the heavy chains of mouse IgG, and the light chains common to the majority of mouse immunoglobulins. This antibody has not been cross adsorbed against other mouse immunoglobulins, and has been found to react substantially with antibody light chains common to all mouse antibodies. Therefore, significant cross reactivity to non-IgG primary antibodies may occur. No antibody reactivity was detected against non-immunoglobulin mouse serum proteins. This antibody may cross-react with immunoglobulins from other species.
Storage Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.
PrecautionsGoat anti Mouse IgG Antibody (H/L) - HRP is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD14616
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