|Other Accession||P20759, P20761, P20760|
|Calculated MW||36572 Da|
|Immunogen||Purified rat immunoglobulin G.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ig gamma-2C chain C region, IGG2C|
|Target/Specificity||Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.|
|Storage||Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Precautions||Anti Rat IgG Antibody - HRP is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.J Immunol Methods. 360 (1-2): 30-8. 2. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.Br J Cancer. 106 (5): 883-8. 3. Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.Biol Trace Elem Res. 164 (2): 205-11. 4. Zschemisch NH et al. (2016) Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.PLoS One. 11 (5): e0155821.
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