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Histar Detection System

     
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD276 (HCA143) on fomalin fixed, paraffin embedded prostate chippings followign heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection system (ABD14622)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD127 (HCA147) on acetone fixed human tonsil using the Histar detection kit (ABD14622)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human PINK1 (HCA150) on formalin fixed, paraffin embedded human testis following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (ABD14622)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human placenta following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR300A)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human breast cancer tissue folowing heat mediated antigen retrieval with EDTA, pH8.0 using the Histar detection kit (ABD14622)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human chromogranin A (MCA5696) in an immunohistochemical analysis of formalin fixed, paraffin embedded human pancreas following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (ABD14622)
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (ABD14622), original magnification x100
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (ABD14622), original magnification x200
  • IHC - Histar Detection System  ABD14622
    Detection of Mouse anti Human prostate specific antigen (MCA5843G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (ABD14622), original magnification x100
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Shelf Life 6 months from the date of despatch.6 months from the date of despatch.Please see label for expiry date.
Target/Specificity Intended UsePack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining. The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only. All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the"Instructions For Use"link. The procedure has been briefly summarized below: 1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary. 2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS). 3) Apply serum block and incubate for 15 minutes. 4) Apply primary antibody and incubate for 30 minutes. 5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash). 6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash). 7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.[Discard unused substrate solution] 8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.Product FormReconstitutionPreparationPreservative StabilisersPurityApprox. Protein ConcentrationsReagents In The KitPack Size: 50 TestsVIAL 1, Serum Block 1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)VIAL 2, Reagent Boost 1 x 5mlVIAL 3, HRP Polymer 1 x 5mlVIAL 4, Peroxide Blocking Reagent 1 x 5ml, 0.03% Hydrogen Peroxide (H202) VIAL A, DAB Chromogen 1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer VIAL B, DAB Substrate Buffer 4 x 6ml, 0.1% H2O2 in Citric acid bufferPack Size: 150 TestsVIAL 1, Serum Block 1 x 15mlVIAL 2, Reagent Boost 1 x 15mlVIAL 3, HRP Polymer 1 x 15mlPack Size: 500 TestsVIAL 1, Serum Block 1 x 50mlVIAL 2, Reagent Boost 1 x 50mlVIAL 3, HRP Polymer 1 x 50mlPack Size: 50 TestsVIAL 1, Serum Block 1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)VIAL 2, Reagent Boost 1 x 5mlVIAL 3, HRP Polymer 1 x 5mlVIAL 4, Peroxide Blocking Reagent 1 x 5ml, 0.03% Hydrogen Peroxide (H202) VIAL A, DAB Chromogen 1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer VIAL B, DAB Substrate Buffer 4 x 6ml, 0.1% H2O2 in Citric acid bufferPack Size: 150 TestsVIAL 1, Serum Block 1 x 15mlVIAL 2, Reagent Boost 1 x 15mlVIAL 3, HRP Polymer 1 x 15mlPack Size: 500 TestsVIAL 1, Serum Block 1 x 50mlVIAL 2, Reagent Boost 1 x 50mlVIAL 3, HRP Polymer 1 x 50mlPack Size: 50 TestsVIAL 1, Serum Block 1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)VIAL 2, Reagent Boost 1 x 5mlVIAL 3, HRP Polymer 1 x 5mlVIAL 4, Peroxide Blocking Reagent 1 x 5ml, 0.03% Hydrogen Peroxide (H202) VIAL A, DAB Chromogen 1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer VIAL B, DAB Substrate Buffer 4 x 6ml, 0.1% H2O2 in Citric acid bufferPack Size: 150 TestsVIAL 1, Serum Block 1 x 15mlVIAL 2, Reagent Boost 1 x 15mlVIAL 3, HRP Polymer 1 x 15mlPack Size: 500 TestsVIAL 1, Serum Block 1 x 50mlVIAL 2, Reagent Boost 1 x 50mlVIAL 3, HRP Polymer 1 x 50mlPreparing The AntibodyTest PrincipleBuffer SolutionAdditional Reagents Required1) Primary antibody and positive control 2) Phosphate Buffered Saline (PBS), pH7.4 (+ 0.2) 3) Counterstain 4) Mounting media Bio-Rad can supply a number of these products separately, please see the ‘Recommended Useful Reagents’ section. StorageStore at +4oC. DO NOT FREEZE.This product should be stored undiluted.Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.Shelf Life6 months from the date of despatch.6 months from the date of despatch.Please see label for expiry date.AcknowledgementsRegulatoryFor research purposes only
Storage Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.
PrecautionsHistar Detection System is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Nishimoto, K. et al. (2010) Association of EMCN with Susceptibility to Rheumatoid Arthritis in a Japanese Population.
J Rheumatol. 38: 221-8. 2. Dedrick, G.S. et al. (2011) Immunohistochemical study of human costotransverse joints: A preliminary investigation.
Clin Anat. 24 (6): 741-7. 3. Alexopoulou, Z. et al. (2016) Deubiquitinase Usp8 regulates a-synuclein clearance and modifies its toxicity in Lewy body disease.
Proc Natl Acad Sci U S A. Jul 21. pii: 201523597. [Epub ahead of print]

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