|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Intended UseProduct FormStreptavidin conjugated to R-Phycoerythrin - lyophilisedReconstitutionReconstitute with 1ml distilled waterPreparationPure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.STAR4A conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum AlbuminPurityApprox. Protein ConcentrationsReagents In The KitPreparing The AntibodyTest PrincipleBuffer SolutionPhosphate buffered salineStoragePrior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life12 months from date of reconstitution.AcknowledgementsRegulatoryFor research purposes only|
|Storage||Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend|
|Precautions||Streptavidin conjugated to R-Phycoerythrin - lyophilised is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. van den Nieuwenhof, I.M. et al. (2001) Differential galactosylation of neuronal and haematopoietic signal regulatory protein-alpha determines its cellular binding-specificity.J Cell Sci. 114 (Pt 7): 1321-9. 2. Tasker, L. & Marshall-Clarke, S. (1997) Immature B cells from neonatal mice show a selective inability to up-regulate MHC class II expression in response to antigen receptor ligation.Int Immunol. 9 (4): 475-84. 3. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.J Immunol. 181: 6514-24. 4. Guesdon, F. et al. (2012) Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling.Biosci Rep. 32 (6): 653-60.
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