|Application ||WB, E|
|Calculated MW||35404 Da|
|Purification||Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Purified rabbit IgG from normal rabbit serum.|
|Shelf Life||18 months from date of despatch|
|Other Names||Ig gamma chain C region, IGHG|
|Target/Specificity||Sheep anti Rabbit IgG antibody conjugated to HRP recognizes rabbit IgG as demonstrated by ELISA and double diffusion.Cross-reactivity against human, mouse, rat and bovine immunoglobulins is reduced by solid phase adsorption.|
|Storage||Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend|
|Precautions||Anti Rabbit IgG (Multi Adsorbed) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Teupser, D. et al. (2004) Major reduction of atherosclerosis in fractalkine (CX3CL1)-deficient mice is at the brachiocephalic artery, not the aortic root.Proc Natl Acad Sci U S A. 101 (51): 17795-800. 2. Wang, Z. et al. (2008) Acute hypercapnia improves indices of tissue oxygenation more than dobutamine in septic shock.Am J Respir Crit Care Med. 177: 178-83. 3. Su, F. et al (2007) Beneficial effects of ethyl pyruvate in septic shock from peritonitis.Arch Surg.142: 166-71. 4. Steinhoff-Wagner, J. et al. (2015) Ontogenic Changes of Villus Growth, Lactase Activity, and Intestinal Glucose Transporters in Preterm and Term Born Calves with or without Prolonged Colostrum Feeding.PLoS One. 10 (5): e0128154. 5. Bonda, T.A. et al. (2013) CCN1 expression in interleukin-6 deficient mouse kidney in experimental model of heart failure.Folia Histochem Cytobiol. 51 (1): 84-91. 6. Makuszewska M et al. (2015) Enhanced expression of hepatocyte growth factor in the healing of experimental acute tympanic membrane perforation.Int J Pediatr Otorhinolaryngol. 79 (7): 987-92. 7. Silvestre FT et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.J Dairy Sci. 94 (5): 2285-301. 8. Savard, A. et al. (2013) Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.J Neuroinflammation. 10: 110. 9. Dreesen, I.A. & Fussenegger, M. (2011) Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells.Biotechnol Bioeng. 108 (4): 853-66. 10. Dreesen, I.A. et al. (2010) Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureusinfection.J Biotechnol. 145 (3): 273-80. 11. Kim, T. et al. (2012) Genetically programmed superparamagnetic behavior of mammalian cells.J Biotechnol. 162 (2-3): 237-45. 12. Folcher, M. et al. (2013) Synthetic mammalian trigger-controlled bipartite transcription factors.Nucleic Acids Res. 41 (13): e134. 13. Müller M et al. (2012) A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine.Metab Eng. 14 (4): 325-35. 14. Bonda, T.A. et al. (2012) Atrial expression of the CCN1 and CCN2 proteins in chronic heart failure.Folia Histochem Cytobiol. 50 (1): 99-103. 15. Šunderić, M. et al. (2015) Lectin-based protein microarray analysis of differences in serum alpha-2-macroglobulin glycosylation between patients with colorectal cancer and persons without cancer.Biotechnol Appl Biochem. Jun13 [Epub ahead of print] 16. Tastanova, A. et al. (2016) Overexpression of YY1 increases the protein production in mammalian cells.J Biotechnol. 219: 72-85. 17. Bonda, T.A. et al. (2016) Interleukin 6 modulates PPARα and PGC-1α and is involved in high-fat diet induced cardiac lipotoxicity in mouse.Int J Cardiol. 219: 1-8. 18. Penezić, A. et al. (2017) A microscale protocol for the isolation of transferrin directly from serum.Clin Chim Acta. 471: 12-16.
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