|Other Accession||P20761, P20759, P20760|
|Calculated MW||36572 Da|
|Purification||Antisera to rat IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ig gamma-2C chain C region, IGG2C|
|Target/Specificity||FITC conjugated Goat (F(ab′)2 anti Rat IgG antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.|
|Storage||Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature th|
|Precautions||Anti Rat IgG (Mouse Adsorbed) Antibody - FITC is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fowles, L. et al. (2000) Regulation of urokinase plasminogen activator gene transcription in the RAW264 murine macrophage cell line by macrophage colony-stimulating factor (CSF-1) is dependent upon the level of cell surface receptor. Biochem. J. 347: 313 - 320. 2. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10. Br J Cancer. 106: 883-888 3. Haraoka, M. et al. (1999) Neutrophil recruitment and resistance to urinary tract infection.J Infect Dis. 180: 1220-9. 4. Hang, L. et al. (2000) Interleukin-8 receptor knockout mice have subepithelial neutrophil entrapment and renal scarring following acute pyelonephritis.J Infect Dis. 182: 1738-48. 5. Franke, H. et al. (2005) P2X(7) receptor-mRNA and -protein in the mouse retina; changes during retinal degeneration in BALBCrds mice.Neurochem Int. 47: 235-42. 6. Parish, N.M. et al. (1999) Anti-CD44 treatment does not prevent the extravasation of autopathogenic T cells to the thyroid in experimental autoimmune thyroiditis.Immunology. 97: 533-9. 7. Song, L. et al. (2011) Deletion of the murine scavenger receptor CD68.J Lipid Res. 52: 1542-50. 8. Chapoval, S.P. et al. (2009) Lung vascular endothelial growth factor expression induces local myeloid dendritic cell activation.Clin Immunol. 132: 371-84. 9. Englezou, P.C. et al. (2015) P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages.Cytokine. 74 (2): 293-304.
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