|Other Accession||P01867, P01864, P01869, P01863, P03987, P01868|
|Calculated MW||43949 Da|
|Purification||Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ig gamma-2A chain C region, membrane-bound form, Igh-1a|
|Target/Specificity||Goat anti Mouse IgG antibody recognizes mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues. Goat IgG shows minimal binding to Fc receptors on rat tissue. We recommend diluting this product in buffer containing 10% normal rat serum to remove any residual cross-reactivity.|
|Storage||Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature th|
|Precautions||Anti Mouse IgG (Rat Adsorbed) Antibody - FITC is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Voulgaraki, D. et al. (2005) Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor.Immunology. 115 (3): 337-46. 2. Win, T.S. et al. (2009) Donor CD4 T cells contribute to cardiac allograft vasculopathy by providing help for autoantibody production.Circ Heart Fail. 2 (4): 361-9. 3. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.FASEB J. 26 (1): 51-62. 4. Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.J Immunol. 188 (6): 2643-52. 5. Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.Invest Ophthalmol Vis Sci. 53 (11): 7358-69. 6. Gibbings, D.J. et al. (2007) CD8α is expressed by human monocytes and enhances FcγR-dependent responses.BMC Immunol. 8: 12. 7. Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.J Genet Genomics. 42 (8): 437-44. 8. Strazielle, N. et al. (2016) T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium.PLoS One. 11 (3): e0150945. 9. Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print] 10. Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.Cell Rep. 15 (6): 1214-27.
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