|Other Accession||P20760, P20762, P20759|
|Calculated MW||36497 Da|
|Purification||Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.|
|Immunogen||Whole rat IgG.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Ig gamma-2B chain C region, Immunoglobulin heavy chain 1a, Igh-1a|
|Target/Specificity||Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues. We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.|
|Storage||Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.This product should be stored undiluted.DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend|
|Precautions||Anti Rat IgG (Mouse Adsorbed) Antibody - RPE is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.J. Exp. Med. 196:1515-1521. 2. Broderick, C.A. et al. (2005) Local Administration of an Adeno-associated Viral Vector Expressing IL-10 Reduces Monocyte Infiltration and Subsequent Photoreceptor Damage during Experimental Autoimmune UveitisMol Ther. 12: 369-73. 3. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell–endothelium interactionsJ Anat. 214: 183-95. 4. Curran, C.S. et al. (2006) Lactoferrin activates macrophages via TLR4-dependent and -independent signaling pathwaysCell Immunol. 242: 23-30. 5. Guilloteau, L.A. et al. (2003) Nramp1 Is Not a Major Determinant in the Control of Brucella melitensis Infection in MiceInfect Immun. 71: 621-8. 6. Maemura, K. et al. (2006) Antigen-presenting cells expressing glutamate decarboxylase 67 were identified as epithelial cells in glutamate decarboxylase 67-GFP knock-in mouse thymus.Tissue Antigens. 67: 198-206.
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