|Application ||WB, IHC, E|
|Preparation||Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human LEC as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein G affinity chromatography.|
|WesternBlot||To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human LEC when used with BioGems' biotinylated antigen affinity purified anti-human LEC (60-203BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.|
|Immunohistochemistry||To yield one-half maximal inhibition [ND50] of the biological activity of Human LEC (100 ng/ml), a concentration of 1.0-5.0 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human LEC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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