|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure recombinant Human MIP-3α. Anti-Human MIP-3α specific antibody was purified by affinity chromatography employing immobilized Human MIP-3α matrix.|
|WesternBlot||To detect HumanMIP-3α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Human MIP-3α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIP-3α (60-227BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3α.|
|Immunohistochemistry||This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human MIP-3α Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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