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Anti-Human MIP-3α Antibody
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Application 
| WB, E |
|---|---|
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Preparation | Produced in mice using highly pure recombinant human MIP-3α as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography. |
|---|---|
| WesternBlot | To detect Human MIP-3α by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3α is 0.25-0.50 ng/lane, under non-reducing conditions. |
| Sandwich | In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3α when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3α (60-227BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human MIP-3α Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
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Application Protocols
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$ 216.00
Cat# ABG10417-U500
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